Fig. 5. PVT1 regulated the stemness and TMZ resistance of glioma cells through miR-365/ELF4/SOX2 pathway. (A) The expression level of ELF4 was downregulated by PVT1 knockdown, which was rescued by miR-365 inhibitor, as detected by RT-qPCR. (B) Cell proliferation was inhibited by PVT1 knockdown, which was rescued by miR-365 inhibitor, as indicated by CCK8. (C) The protein level of ELF4 and stemness factors (SOX2, Oct4, Nanog) were inhibited by PVT1 knockdown, which was rescued by miR-365 inhibitor in glioma cells, as shown by western blot. GAPDH was used for normalization. (D) PVT1 knockdown reduced glioma cell viability and sensitized cells to TMZ, which was rescued by miR-365 inhibitor, as measured by CCK8 assay. Data are presented as Mean±SD of three independent experiments. *p<0.05, **p<0.01.
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