Used antibodies in this study
Antibodies | Dilution factors for | |
---|---|---|
Western blot | Immunostaining | |
Mouse anti-PSD95 (abcam, ab2723) | 1:2,000 | |
Mouse anti-gapdh (Santacruz, sc32233) | 1:2,000 | |
Rabbit anti-GFAP (Sigma, G9269) | 1:500 | |
Rabbit anti-NeuN (Millipore, ABN78) | 1:2,000 | |
Rabbit anti-Iba-1 (Wako, 019-19741) | 1:1,000 | |
Rabbit anti-synaptophysin (abcam, ab32127) | 1:5,000 | |
Rat anti-CD45 (Mybiosource, MBS520149) | 1:1,000 | |
Donkey anti-mouse IgG, Alexa Fluor 488 (Thermo Fisher scientific, A-21202) | 1:500 | |
Donkey anti-mouse IgG, Alexa Fluor 647 (Thermo Fisher scientific, A-31571) | 1:500 | |
Donkey anti-rabbit IgG, Alexa Fluor 647 (Thermo Fisher scientific, A-31573) | 1:500 | |
Donkey anti-rat IgG, Alexa Fluor 488 (Thermo Fisher scientific, A-21208) | 1:500 | |
Goat anti-mouse IgG (H+L) secondary antibody HRP (Thermo Fisher scientific, 31431) | 1:20,000 | |
Goat anti-rabbit IgG (H+L) secondary antibody HRP (Thermo Fisher scientific, 31460) | 1:20,000 |