Fig. 2. iPSCs derived from all three groups successfully differentiated into the three germ layers in vitro. (A) Schematic diagram shows the detailed procedure to induce spontaneous iPSC differentiation in vitro. (B) Immunofluorescence labeling data show that EBs spontaneously differentiated from the representative iPSC clones from a normal individual (Normal 1-5), DMD patient without ID (DMD 3-5), and DMD patient with ID (DMD 1-5) were positively labeled GATA4 (endoderm), SMA (mesoderm), NESTIN and SOX2 (ectoderm). Scale bar, 50 μm.
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