Fig. 1. Distribution of laminin and collagen type IV in the SVZ of an adult mouse brain. (A) Numerous speckle-shaped fractones are located on the ependymal layer in the lateral ventricle and 3rd ventricle, with the exception of the 4th ventricle. Speckle-shaped fractones are stained with laminin (green), and ependymal cells are stained with S100ß (red). Scale bar, 100 μm. Scale bar in the inset 10 μm. A, anterior; P, posterior; D, dorsal; V, ventral. (B) A 3D view of the SVZ region in a whole-mount preparation of the adult mouse brain. Scale bar, 30 μm. High magnified image of speckle-shaped fractones in the lateral ventricle ependymal layer whole-mount view (C), coronal sectioned view (D). Scale bar, 10 μm. (E) Comparison of vascular architectures in the SVZ and cortex using the z-projection. Scale bar, 20 μm (F) A dot plot showing signal intensities of laminin and collagen type IV in the microvessels of SVZ and cortex. The best-fit linear regression line for SVZ is in red, and cortex in black. The intercepts and the slopes are presented for each brain region (F=12, p=0.0005). (G) A boxplot showing the ratios between laminin (LN) and collagen type IV (CIV) in blood vessels of SVZ and cortex. ****p<0.0001. (H) Laminin and collagen type IV are distributed in different patterns along the branches of the vascular tree in the anterior and ventral parts of the SVZ. Transit-amplifying cells stained with EGFR (blue) are located in close proximity to laminin-rich blood vessels. Scale bar, 50 μm. SVZ, subventricular zone; EGFR, epidermal growth factor receptor.
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