Fig. 3. ECM preference of adult NS/PCs using cell micropatterning. (A) Schematic diagram of the micropattern assay. One substrate was applied on the coverglass, and another substrate was loaded on a PDMS microstamp and stamped onto the coated coverglass. (B) Representative images of cell micropatterning using collagen type IV (C IV), laminin (LN), and PLO (P) as control. SVZ-derived progenitors preferred to adhere to the patterned substrate, with the exception of cells on the laminin-coated coverglass. Cells exhibited stronger adherence to laminin over collagen type IV, and the addition of collagen type IV on laminin decreased the adhesive effect of laminin (right). Scale bar, 50 μm. (C, D) The percentage of cells attached to patterned or coated substrates determined by the positioning of their nuclei and process alignment on the micropatterned area. Data represent mean and SD from at least three independent experiments. **p<0.01, n.s, >0.05. ECM, extracellular matrix; PDMS, polydimethylsiloxane; PLO, polyornitine; SVZ, subventricular zone; SD, standard deviation.
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