Fig. 4. Perturbation of ECM preference by SDF1 treatment and functional blocking of α6 integrin. (A) Representative images of cell micropatterning. SVZ-derived progenitors were seeded on laminin pattern on collagen type IV coating (LN on C IV; upper panel) and collagen type IV pattern on laminin coating (C IV on LN; lower panel). SDF1 was added to the cultured medium, and cells were pretreated with GoH3 (blocking antibody for α6 integrin) prior to the micropattern assay. SDF1 eliminated the inhibitory effects of collagen type IV on laminin, whereas GoH treatment perturbed the laminin preference of adult NS/PCs. Scale bar, 50 μm. (B,C) The percentage of cells attached to patterned or coated substrates determined by nuclei positioning and process alignment. Data represent mean and SD from at least three independent experiments. *p<0.05, **p<0.01, ****p<0.0001, n.s, >0.05. ECM, extracellular matrix; SVZ, subventricular zone; SDF1; stromal-derived factor 1; NS/PCs, neural stem/progenitor cells; SD, standard deviation.
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