Fig. 3. Hyperoxygenation reduced inflammatory responses in the hippocampus of Tg-APP/PS1 mice. (A, B) Immunohistochemical images showing GFAP-stained astroglia in the dentate gyrus (A) of wildtype control (WT-CON), wildtype mice exposed to hyperoxygenation (WT-HO2), Tg-APP/PS1 control mice (Tg-CON), and Tg-APP/PS1 mice exposed to hyperoxygenation (Tg-HO2). Bottom panels, high magnification of the boxed areas. GFAP, red; DAPI, blue. Scale bar, 200 μm. Quantification of the levels by total stained area and stained intensity (B). n=7~9 animals/group, 4~8 repeats. (C, D) Immunohistochemical images showing Iba-1-stained microglia in the dentate gyrus (C) of WT-CON, WT-HO2, Tg-CON, and Tg-HO2 mice. Bottom panels, high magnification of the boxed areas. Iba-1, red. Scale bar, 200 μm. Quantification levels by total stained area (D). n=7~9 animals/group, 4~8 repeats. (E, F) Photomicrographs showing thioflavin S (ThS)-stained plaques (top panels, green) and Iba-1-stained microglia images (bottom panels, red) merged with ThS (green)-stained plaque images in the dentate gyrus (E) of WT-CON, WT-HO2, Tg-CON, and Tg-HO2 mice. Scale bar, 200 μm. Quantification levels of Iba-1-stained areas surrounding ThS-stained plaques (F). n=8~9 animals/group, 4~6 repeats. (G, H) Western blot data showing expression levels of p-NFkB, NF-kB (p65), p-p38, p38, GFAP, and Iba-1 in the hippocampus (G) and prefrontal cortex (H) of WT-CON, WT-HO2, Tg-CON, and Tg-HO2 mice, and their quantification levels. n=6~8 animals/group, 5~10 repeats. Data are presented as mean±SEM. *,**difference between indicated groups. *p<0.05; **p<0.01 (two-way ANOVA and Bonferroni post hoc test).
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