Experimental Neurobiology

List of primers used

scRT-PCR

Target gene	Outer primer pairs	Inner primer pairs	GenBank no.

Gapdh (268, 127 bp)	(F) 5’-GTTCCTACCCCCAATGTGTCC-3’	(F) 5’-TTGTGATGGGTGTGAACCAC-3’	NM_001289726.1, NM_008084.3
	(R) 5’-ACCTGTTGCTGTAGCCGTATC-3’	(R) 5’-CCTTCCACAATGCCAAAGTTG-3’
Gfap (350, 160 bp)	(F) 5’-TGCGTATAGACAGGAGGCAGA-3’	(F) 5’-TGGAGGAGGAGATCCAGTTCTT-3’	NM_001131020.1, NM_010277.3
	(R) 5’-GGCGATAGTCGTTAGCTTCGT-3’	(R) 5’-GCCACTGCCTCGTATTGAGT-3’
Nefh (285, 152 bp)	(F) 5’-GCAGCCAAAGTGAACACAGA-3’	(F) 5’-AGAGTTGGAGGCCCTGAAAA-3’	NM_010904.3
	(R) 5’-AATGTCCAGGGCCATCTTGA-3’	(R) 5’-GTGCAGCCATCTCCCACTT-3’
Tlr2 (305, 118 bp)	(F) 5’-TCTCTGGGCAGTCTTGAACA-3’	(F) 5’-AGACACTGGGGGTAACATCG-3’	NM_011905.3
	(R) 5’-TCGCGGATCGACTTTAGACT-3’	(R) 5’-AGAGAAGTCAGCCCAGCAAA-3’

Real-time RT-PCR

Target gene	Primer pairs		GenBank no.

Gapdh (282 bp)	(F) 5’-CCAGAACATCATCCCTGCAT-3’ (R) 5’-GCATCGAAGGTGGAAGAGTG-3’		NM_001289726.1, NM_008084.3
Myd88 (101 bp)	(F) 5’-AAGGAATGTGACTTCCAGACCA-3’ (R) 5’-AAGTCCTTCTTCATCGCCTTGT-3’		NM_ 010851.3
Il1b (122 bp)	(F) 5’-GGCTGCTTCCAAACCTTTGAC-3’ (R) 5’-AGCTTCTCCACAGCCACAAT-3’		NM_008361.4
Tlr1 (106 bp)	(F) 5’-TCAAGTGTGCAGCTGATTGCT-3’ (R) 5’-TGCTAACGTGCCGAAGAGATC-3’		NM_001276445.1, NM_030682.2
Tlr2 (110 bp)	(F) 5’-CCTCTATTCCCTCCGGACTC-3’ (R) 5’-AACCTGGAGGTTCGCACA-3’		NM_011905.3
Tlr4 (103 bp)	(F) 5’- AGTGGCCCTACCAAGTCTCA-3’ (R) 5’- GTGTCTCAGGCTGTTTGTTCC-3’		NM_021297.3
Tlr6 (139 bp)	(F) 5’-TGAGCCAAGACAGAAAACCCA-3’ (R) 5’-GGGACATGAGTAAGGTTCCTGTT-3’		NM_001384171.1, NM_011604.5, NM_001359180.2
Tnf (100 bp)	(F) 5’-AGGTTCTCTTCAAGGGACAAGG-3’ (R) 5’-GAGAGGAGGTTGACTTTCTCCTG-3’		NM_013693.3, NM_001278601.1

Gapdh, Glyceraldehyde-3-phosphate dehydrogenase; Gfap, Glial fibrillary acidic protein; Nefh, Neurofilament heavy chain; Myd88, myeloid differentiation primary response gene 88; Il1b, Interleukin 1 Beta; Tlr1, Toll-like receptor 1; Tlr2, Toll-like receptor 2; Tlr4, Toll-like receptor 4; Tlr6, Toll-like receptor 6; Tnf, Tumor necrosis factor.

Exp Neurobiol 2021;30:329~340 https://doi.org/10.5607/en21018