Fig. 2. The effects of Pam2CSK4 on [Ca2+]i responses in TG neurons. (A, B) Representative traces (different colors indicate three individual neurons, A) and quantitative data of reproducible [Ca2+]i responses induced by Pam2CSK4 (1 µg/ml, 100 s/application) in TG neurons (n=11 neurons from n=2 mice; one-way ANOVA, p=0.5022, ns, B). (C) Representative traces showing Pam2CSK4 (1 µg/ml, 100 s)-induced [Ca2+]i responses in TRPV1-positive (a blue trace) and TRPV1-negative (a black trace) TG neurons. (D) The percentage of CAP-positive (50%, n=22 of 44) and CAP-negative neurons (50%, n=22 of 44) in Pam2CSK4 responding neurons. n=4 mice. (E) Representative traces (different colors indicate three individual neurons) showing the complete abolition of the Pam2CSK4-induced [Ca2+]i responses in TLR2 KO mice. (F) The percentage of Pam2CSK4-responding neurons in the WT mice (approximately 8.7%, n=11 of 126, used the same data with panel B) is significantly reduced in TLR2 KO mice (0%, n=0 of 76 KCl-responding neurons from n=1 mouse; unpaired Student t-test, *p=0.0112). The data are expressed as the mean±SEM. ns, not significant.
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