Fig. 3. Tlr2 mRNA expression in dental primary afferent (DPA) neurons. (A) A schematic for collecting DPA neurons labeled by DiI (black arrows) for use with single-cell reverse transcriptase polymerase chain reaction (scRT-PCR). Scale bar, 40 µm. (B) Representative gels showing scRT-PCR products indicating mRNA expression of Tlr2 (118 bp), Nefh (152 bp), Gfap (160 bp), and Gapdh (127 bp) from a total of nine DPA neurons. (C) Quantitative scRT-PCR data showing the size distributions of six Tlr2-positive neurons amongst a total of 35 DPA neurons. n=8 mice.
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