Fig. 3. Restraint stress did not change the excitatory input to NPY neurons. (A) Schematic diagram for whole cell patch-clamp for EPSCs. (B) Representative traces of spontaneous excitatory postsynaptic currents (sEPSC) in NPY neurons from CON and CRS mice. sEPSC was confirmed to block the excitatory signals by bath application of CNQX and APV (top). The gray box in left traces were enlarged to right traces. B. The frequency of sEPSC was not affected by the restraint stress. (C) The amplitude of sEPSC was not changed by restraint stress. (D) The amplitude of sEPSCs. (E) The area of sEPSCs. (F) The rise time of sEPSCs. (G) The decay time of sEPSCs. (H) Schematic diagram for whole cell patch-clamp for spontaneous inhibitory postsynaptic currents (sIPSCs). (I) Representative traces of sIPSCs in POMC neurons from CON and CRS group. The application of bicuculline (Bic) was conducted to confirm sIPSCs (top). Enlarged traces (gray box) represent to right traces. (J) The frequency of sIPSC. (K) The amplitude of sIPSC. (L) The area of sIPSCs. (M) The rise time of sIPSCs. (N) The decay time of sIPSCs. The data for sEPSC were from CON (12 neurons from 7 mice) and CRS (19 neurons from 12 mice). The data for sIPSCs were from CON (8 neurons from 3 mice) and CRS (13 neurons from 4 mice).
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