Fig. 1. OGD/R treatment leads to hippocampal neuron pyroptosis. An OGD/R cell model was established in primary hippocampal neurons. (A) Pyroptosis was detected by flow cytometry using caspase-1 and PI staining. (B) ELISA was applied to measure IL-1β and IL-18 expression. (C) LDH release in the cell supernatant. (D) Pyroptosis-related markers, including NLRP3, pro-caspase-1, cleaved caspase-1, GSDMD-FL, and GSDMD-N, were assessed using western blot assay. The results are shown as the mean±SD. n=3 per group. *p<0.05, **p<0.01, ***p<0.001.
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