Fig. 4. miR-186-5p knockdown alleviates OGD/R-induced pyroptosis. The miR-186-5p inhibitor or inhibitor NC was transfected into neurons. (A) Expression of miR-186-5p was measured using qRT-PCR. (B) Pyroptosis was assessed using flow cytometry. (C) Expression of IL-1β and IL-18 was detected by ELISA. (D) LDH release was restored by inhibition of miR-186-5p. (E) Pyroptosis-related markers were detected by western blot assay. The results are shown as the mean±SD. n=3 per group. *p<0.05, **p<0.01, ***p<0.001.
© Exp Neurobiol
