Fig. 5. miR-186-5p overexpression inhibits Ngb expression. (A) qRT-PCR was used to evaluate the level of miR-186-5p after neurons were incubated with miR-186-5p mimics. (B, C) mRNA (B) and protein (C) levels of Ngb were detected by qRT–PCR and western blotting in neurons. (D) The binding site was predicted using TargetScan version 7.2, and luciferase activity was measured using double luciferase reporter assay in hippocampal neurons. The results are shown as the mean±SD. n=3 per group. *p<0.05, **p<0.01, ***p<0.001.
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