The primer sequences
| Primers | Forward sequence | Reverse sequence |
|---|---|---|
| COX-2 | 5'-CGG AGG AGA AGT GGG GTT TA-3' | 5'-TGG GAG GCA CTT GCG TTC AT-3' |
| GAPDH | 5'-GGG GGC TCT CTG CTC CTC CC-3' | 5'-CGG CCA AAT CCG TTC ACA CCG-3' |
| IL-1β | 5'-CCC TGC AGC TGG AGA GTG TGG-3' | 5'-TGT GCT CTG CTT GAG AGG TGC-3' |
| TNF-α | 5'-CGT CGT AGC AAA CCA CCA AG-3' | 5'-CAC AGA GCA ATG ACT CCA AA-3' |
COX-2, cyclooxygenase-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1β, interleukin-1 beta; TNF-α, tumor necrosis factor-alpha.