Fig. 4. Elevated neuronal GAT1, not astrocytic GAT3, in the ARC after chronic HFD feeding. (A, B) Representative traces of NPA-induced current in Chow and HFD mice. (C) Summarized bar graph showing NPA-induced current density (pA/pF) in Chow and HFD mice (Chow, n=8 cells from 3 mice; HFD, n=8 cells from 3 mice; Mann-Whitney test, p=0.0281). (D, E) Representative traces of NO711-induced current in Chow and HFD mice. (F) Summarized bar graph showing NO711-induced current density (pA/pF) in Chow and HFD mice (Chow, n=9 cells from 3 mice; HFD, n=9 cells from 3 mice; Mann-Whitney test, p=0.0315). (G, H) Representative traces of SNAP5114-induced current in Chow and HFD mice. (I) Summarized bar graph showing SNAP5114-induced current density (pA/pF) in Chow and HFD mice (Chow, n=8 cells from 2 mice; HFD, n=8 cells from 3 mice; Mann-Whitney test, p=0.3823). (J) Representative images of GAT1 and NeuN immunostaining in the ARC of Chow and HFD mice. (K) Quantification of neuronal GAT1 intensity in Chow and HFD mice (Chow, n=212 cells from 3 mice; HFD, n=300 cells from 3 mice; Mann-Whitney test, p=0.0003). (L) Representative images of GAT3 and GFAP immunostaining in the ARC of Chow and HFD mice. (M) Quantification of astrocytic GAT3 intensity in Chow and HFD mice (Chow, n=40 cells from 3 mice; HFD, n=51 cells from 3 mice; Mann-Whitney test, p=0.064). Data are presented as mean±SEM. *p<0.05; ***p<0.001.
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