Fig. 2. The analysis of circadian oscillations of Bmal1 and Per2 in the cortex and the hippocampus. (A) The qRT-PCR (left) and western blot (right) of the cortex demonstrate a comparable pattern of mRNA oscillations in Bmal1 and Per2 between wild-type and P301S mice. The altered expression of Bmal1 protein is noted at ZT14 and ZT20 compared with the wild-type. (B) The qRT-PCR (left) and western blot (right) of the hippocampus reveals a disruption in circadian oscillations of Bmal1 in both transcript and protein levels in P301S mice. The mRNA level of Per2 was lower at ZT2 and ZT20 in P301S than in wild-type mice, but its overall pattern of oscillations and the protein levels are similar to those of wild-type mice. (A, B) Data are mean±SEM (n=3). Differences were detected with the Mann-Whitney test. *p<0.05, **p<0.01.
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