Fig. 4. The analysis of tau protein under the modulation of Hsp70 in vitro. (A) Markedly reduced tau protein levels both in phosphorylated- and total form are clearly noted after the co-expression of Hsp70 in all tested fractions, soluble, insoluble and whole fractions. Data are mean±SEM (n=3). *p<0.05 and **p<0.01 on Mann-Whitney test. (B) The mRNA levels of tau are not affected by Hsp70 co-expression. Data are mean±SEM (n=3). The ‘ns’ means that the comparison was insignificant at the level of p<0.05 on Mann-Whitney test. (C) The representative photos of immunocytochemistry for Hsp70 (green) and tau (red) show nice co-localizations under the overexpression of Hsp70 (right). Scale bars are 100 μm or 10 μm as indicated. (D) The western blots show a level change of the indicated proteins according to the ZT after the application of CQ (left) and MG132 (right). The decreased total tau proteins by Hsp70 overexpression are partly reversed by CQ and MG 132 treatment. EV, empty vector; FC, fold-change; ns, non-significance.
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