Fig. 4. Kv1.1 and Kv1.2-immunoreactivity in dendritic terminals and cell bodies of ANFs. (A~C) Low magnification confocal micrographs of whole-mount cochlear apical turn, double-labeled with anti-Kv1.1 (A, red) and anti-calretinin (B, green). Kv1.1 immunoreactivity is found near IHCs (yellow arrow) and in the spiral ganglion. Scale bar: 100 μm. (D~F) High-magnification confocal images of spiral ganglion. Double-labeling with a monoclonal anti- Kv1.1 (D, green) and anti-NKA (E, red) shows that Kv1.1 immunoreactivity is detected in the cell membranes and the cytosols of the majority of NKA-positive spiral ganglion cells. Scale bar: 10 μm. (G~I) Low magnification confocal micrographs of whole-mount cochlear apical turn, double-labeled with anti-Kv1.2 (G, red) and anti-calretinin (H, green). Kv1.2 immunoreactivity is found near IHCs (yellow arrow) and in the spiral ganglion. Scale bar: 150 μm. (J~L) High-magnification confocal images of spiral ganglion. Double-labeling with a monoclonal anti- Kv1.2 (J, red) and anti-NKA (K, green) shows that Kv1.2 immunoreactivity is detected in the cell membranes of the majority of NKA-positive spiral ganglion cells. Scale bar: 10 μm.
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