Fig. 7. Kv1.1 and Kv1.2 immunoreactivities are abundant at the first heminodes of ANFs. (A~C) Confocal micrographs of cochlear preparations double-labeled with anti-Kv1.1 (red), and anti-NKA (green). (D) Intensity profile of Kv1.1 and NKA-immunoreactivity along the length of peripheral process of ANF shown in A~C. (E~H) Confocal micrographs of cochlear preparations triple-labeled with anti-Kv1.1 (red), anti-NKA (grey) and anti-caspr-2 (green). (I~K) Confocal micrographs of cochlear preparations double-labeled with anti-Kv1.2 (red), and anti-NKA (green). (L) Intensity profile of Kv1.2 and NKA-immunoreactivity along the length of peripheral process of ANF shown in I~K. (M~P) Confocal micrographs of cochlear preparations triple-labeled with anti-Kv1.2 (red), anti-NFH (grey) and anti-caspr-2 (green). In addition to the high-intensity area at the dendritic segments, another signal peak was detected along the length of peripheral processes of ANFs. The second hot spots were found just below habenula perforata and corresponded well with Caspr-2 signals (dotted box in E~H and M~P). Scale bar: 5 μm.
© Exp Neurobiol
