Fig. 8. Immunolabeling of Kv7.2 and K2P2.1 subunits in whole-mount cochlear preparations. (A~C) Confocal images of cochlear turn double-labeled with anti-Kv7.2 (A, green) and anti-NKA (B, red). Outlines of IHCs are marked with dotted lines. Yellow arrow: Kv7.2 and NKA-positive dendritic terminals. White arrow: Kv7.2-positive but NKA-negative spots. White arrowhead: NKA-positive but Kv7.2-negative terminals. Scale bar: 10 μm. (D~F) Confocal images of spiral ganglion double-labeled with anti-Kv7.2 (D, green) and anti-calretinin (E, red). Kv7.2-immunoreactivity is detected in most calretinin-positive spiral ganglion somata (yellow arrow). Scale bar: 20 μm. (G~I) Confocal images of cochlear turn double-labeled with anti-K2P2.1 (G, Green) and anti-NKA (H, red). Outlines of IHCs are marked with dotted lines. No K2P2.1-immunoreactivity is detected at the NKA-positive dendritic terminals. Scale bar: 10 μm. (J~L) Confocal images of spiral ganglion double-labeled with anti-K2P2.1 (J, green) and anti-NFH (K, red). K2P2.1-immunoreactivity is detected in NFH-positive spiral ganglion somata (yellow arrow). Scale bar: 10 μm.
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