Fig. 1. Reelin modulates dendritic spine formation by enhancing the PSD-95 puncta number in vitro. (A) Primary hippocampal neurons were transfected with GFP plasmid on DIV 19 and treated with vehicle (Mock medium) or Reelin (0.7 or 1.4 μM) on DIV 20 for 24 h. Then, dendritic spine density was measured (vehicle treated: n=18 dendrites from 9 neurons; 0.7 μM Reelin: n=11 dendrites from 9 neurons; 1.4 μM Reelin: n=7 dendrites from 5 neurons). (B) Primary hippocampal neurons were treated as described above and immunostained with an anti-PSD-95 antibody on DIV 21. The staining intensity and puncta numbers were then quantified (PSD-95 intensity, vehicle: n=37 dendrites from 9 neurons, Reelin: n=61 dendrites from 13 neurons; PSD-95 puncta number, vehicle: n=42 dendrites from 9 neurons, Reelin: n=83 dendrites from 13 neurons). *p<0.05, ***p<0.001. Scale bar=10 μm.
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