Fig. 2. Reelin selectively alters NMDA receptor subunit levels in primary hippocampal neurons. (A-C) Primary hippocampal neurons were transfected with GFP plasmid on DIV 19, treated with vehicle (Mock medium) or 1.4 μM Reelin on DIV 20 for 24 h, and immunostained with anti-GluN1, anti-GluN2A, or anti-GluN2B antibodies. Then, the intensities of GluN1 (A, vehicle: n=43 dendrites from 7 neurons, Reelin: n=51 dendrites from 6 neurons), GluN2A (B, vehicle: n=28 dendrites from 6 neurons, Reelin: n=22 dendrites from 5 neurons), and GluN2B (C, vehicle: n=34 dendrites from 5 neurons, Reelin: n=46 dendrites from 5 neurons) were measured. ***p<0.001. Scale bar=10 μm.
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