Fig. 3. Reelin increases AMPAR trafficking during synaptogenesis in primary hippocampal neurons. (A, B) Primary hippocampal neurons were transfected with GFP plasmid on DIV 12 and treated with vehicle (Mock medium) or 1.4 μM Reelin on DIV 13 for 24 h. On DIV 14, live cell surface staining was conducted with antibodies recognizing the N-terminus of GluA1 or GluA2. Then, puncta numbers of GluA1(A, vehicle: n=38 dendrites from 6 neurons, Reelin: n=46 dendrites from 8 neurons) and GluA2 (B, vehicle: n=18 dendrites from 4 neurons, Reelin: n=27 dendrites from 6 neurons) were measured. *p<0.05, **p<0.01. Scale bar=10 μm.
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