Fig. 5. Effect of glutamatergic activation in the aIC on SDV-induced analgesia. (A) Schematic diagram of experiment schedule design. (B) Location of microinjection site in the aIC with atlas of Paxinos and Watson (2nd edition). (C) Representative fluorescence image illustrating expression of c-Fos (red), NeuN (green), and DAPI (blue) in the aIC of formalin-treated SDV group after vehicle or NMDA injection. Scale bar represents 10 μm; magnification 200×. (D) Change in nocifensive behaviors of formalin-treated SDV group with NMDA or vehicle infusion (n=6~7 per group). (E) Comparison for nocifensive behaviors in the formalin-treated SDV group with NMDA or vehicle injection in the second phase of formalin test. Data are presented as mean±SEM. **p<0.01 (one-way ANOVA followed by Bonferroni test).
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