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Fig. 1. Alternative splice variants of ANO2 channels in the brain regions. (A) The predicted transmembrane topology of ANO2 channels. The orange indicates the position of alternative splicing sites in the N-terminal region and first intracellular loop. The putative calcium-binding sites are indicated in pink circles on transmembrane domains 6, 7, and 8. (B) Schematic diagram summarizing the genomic structure of ano2. The orange bars indicate the alternative exons, and the grey bars indicate common exons. (C) RT-PCR analyses of ano2 splice variants of the controls and brain regions using cDNAs from an NIH3T3 cell line (NIH, for a negative control), the olfactory epithelium (OE, for positive control of exon 1b and 4), the retina (RE, for positive control of exon 4 and 14), the cerebral cortex (CX), the hippocampus (HIP), and the thalamus (TH). Expression of the ano2 splice variants containing exons 1a and 1b, exon 2, exon 4, exon 14, and β-actin. (D) Summary diagram of expression of ano2 variants encoding for isoform 1~3 in the control samples and brain regions.
Exp Neurobiol 2023;32:133~146
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