Fig. 1. NAG-1 transgenic mice used in this study. (A) Genotyping of NAG-1 transgenic mice. Genomic DNA was extracted from either wild-type or transgenic mice, and genotyped by PCR and agarose gel electrophoresis. (B) NAG-1 protein expression on various nervous tissues. Immunoblotting confirmed overexpression of NAG-1 on nervous tissues of NAG-1 transgenic mice (+) compared to those of wild-type (-). β-actin was used as loading control. (C) NAG-1 TG mice display lower body weights relative to WT mice. n=6 mice/group. *p<0.05 vs. WT mice. n.s., not significant; N/C, non-template control; TG, transgenic; WT, wild-type.
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