Fig. 4. Graph and photomicrographs illustrating c-Fos immunoreactivity in the ipsilateral and contralateral lumbar spinal cord dorsal horns of female NAG-1 TG and WT mice. (A) The immunofluorescence of c-Fos was quantified in the superficial dorsal horn (SDH, lamina I~II), nucleus proprius (NP, lamina III~IV), and neck region (NECK, lamina V~VI) of the ipsilateral and contralateral lumbar spinal cord dorsal horns in mice. n=6 mice/group. (B) Representative images showing the immunoreactivity of c-Fos in the ipsilateral and contralateral lumbar spinal cord dorsal horns. The spinal cords were sampled at 2 h after formalin injection. Scale bar=200 μm. ***p<0.001 vs. contralateral side. n.s., not significant; TG, transgenic; WT, wild-type.
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