Fig. 4. Psen2 N141I mutation causes selective increase in the production of IL-6 in innate immune cells. (A) Schematic diagram suggesting culture system of primary mouse microglia, astrocytes and bone marrow-derived macrophages. (B~D) ELISA of IL-6 and TNF-α in culture supernatants from WT and KI/+ microglia (B), bone marrow-derived macrophages (BMDM; C), and astrocytes (D) after treatment with the TLR ligands for 12 h (n=4). Cells were stimulated with N-palmitoyl-S-dipalmitoylglyceryl Cys-Ser-(Lys)4 (Pam3CSK4; 100 ng/ml), heat-killed Listeria monocytogenes (HKLM; 107 cells/ml), polyinosinic-polycytidylic acid high molecular weight (poly (I:C) HMW; 10 μg/ml) or low molecular weight (poly (I:C) LMW; 1 μg/ml), lipopolysaccharide (LPS; 1 μg/ml), flagellin from Salmonella typhimurium (FAL-ST; 1 μg/ml), Pam2CGDPKHPKSF (FSL-1; 100 ng/ml), single-strand (ss) RNA (1 μg/ml), or oligonucleotides containing unmethylated CpG dinucleotides (ODN 1826; 0.5 μM). Data are mean±SEM. #p<0.05, ##p<0.01, and ###p<0.001 for the indicated comparisons.
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