Fig. 2. GCaMP6f performance in the mouse anterior cingulate cortex (ACC). (A) Time line of the experiment. Green arrow, virus injection time; Blue line, time period of GA; Red line, sleep deprivation for 72 h; Green line indicates two-photon imaging time course. (B) Cartoon of virus injection sites. (C) Representative micrograph showing the site of GCaMP6f injection into ACC. (D) Schematic of two-photon imaging process. (E) Layer 2/3 cortical neurons imaged in vivo and activity maps. The neurons in the red dot are examples. (F) The spontaneous Ca2+ transients of neurons in (E) maps (scale bar, 10 um).
© Exp Neurobiol
