Fig. 3. GA limited the decrease of Ca2+ transients’ frequency and the underactive neurons fractions at SD stage and remission stage in vivo. (A) Neurons in Layer II/III (300~450 um) of ACC imaged in vivo and activity maps in 0 days before, 3 days SD and one day after SD (from left to right) in SD (top) and GA mice (down), (Red line: The blood vessel, scale bar, 20 um). (B) The overall trend of the average frequency of Ca2+ transients during the whole experiment. Data are shown as mean±SEM., **p<0.01, Two-way ANOVA with Bonferroni’s test. (C) The fractions of <3 Ca2+ transients/min in SD and GA mice. Two-tailed unpaired t-test. 0 d Baseline vs. 3 d SD+Saline, p<0.001; Baseline vs. GA-Baseline, p=0.543; 3 d SD: SD+Saline vs. SD+GA, p=0.012; GA+Baseline vs. 3 d SD+GA, p=0.360. (D) The average frequency of Ca2+ transients in SD 24 h, SD 48 h, SD 72 h, recover 1 d and recover 2 d compare with baseline time 0 d. Two-tailed paired t-test. 0 d Baseline vs. 24 h SD+Saline, p=0.024; 0 d Baseline vs. SD 48 h, p=0.004; 0 d vs. SD 72 h, p<0.001; 0 d vs. Re1d, p=0.019; 0 d vs. Re2d, p=0.082 (E) The average frequency of Ca2+ transients in 0 day, SD 24 h, SD 48 h, SD 72 h, recover 1 d in SD+Saline and SD+GA mice. Two-tailed unpaired t-test. 0 d: Baseline vs. GA+Baseline, p=0.747; SD 24 h: SD+Saline vs. SD+GA, p=0.104; SD 48 h: SD+Saline vs. SD+GA, p=0.315; SD 72 h: SD+Saline vs. SD+GA, p=0.002; recover 1 d: Saline vs. GA, p=0.194. n=42 cells in 6 SD+Saline mice, (F) The average frequency of Ca2+ transients in SD+GA group in SD 24 h, SD 48 h, SD 72 h, recover 1 d and recover 2 d compare with baseline time 0 d. Two-tailed paired t-test. 0 d Baseline vs. 24 h SD+GA, p=0.076; 0 d Baseline vs. 48 h SD+GA, p=0.002; 0 d Baseline vs. 72 h SD+GA, p=0.019; 0 d Baseline vs. Re1d SD+GA, p=0.066; 0 d Baseline vs. Re2d SD+GA, p=0.116, n=95 cells in 5 GA-treated mice. All data are shown as mean±SEM., ***p<0.001, **p<0.01, *p<0.05, ns, not significant. (G) The fractions of <3 Ca2+ transients/min in SD and GA mice. SD :SD+Saline. GA: SD+GA.
© Exp Neurobiol
