Fig. 4. GA modulated the change of calcium transient amplitude induced by SD. (A) Mean calcium transient amplitude of imaged regions daily after GA induction. SD 24 h: SD+Saline vs. SD+GA, p=0.038; SD 48 h: SD+Saline vs. SD+GA, p=0.02. (B~D) The mean amplitude of Ca2+ transients in SD 24 h, SD 48 h, SD 72 h compare with baseline time 0 d. Two-tailed paired t-test. 0 d Baseline vs. SD+Saline 24 h, p=0.039; 0 d Baseline vs. SD+Saline 48 h, p=0.336; 0 d Baseline vs. SD+Saline 72 h, p=0.740. (E~I) The mean amplitude of Ca2+ transients in 0 day, SD 24 h, SD 48 h, SD 72 h, recover 1 d in SD+Saline and SD+GA mice. Kolmogorov-Smirnov test. 0 d: Baseline vs. GA+Baseline, p=0.446; SD 24 h: SD+Saline vs. SD+GA, p=0.038; SD 48 h: SD+Saline vs. SD+GA, p=0.020; SD 72 h: SD+Saline vs. SD+GA, p=0.750; recover 1 d: Saline vs. GA, p=0.699 (J~K) The rise time (J) and fall time (K) of Ca2+ transients during the whole experiment. (L) The rise time of Ca2+ transients in SD 24 h, SD 48 h, SD 72 h in SD+Saline and SD+GA mice. Two-tailed unpaired t-test. SD 24 h: SD+Saline (40 cells) vs. SD+GA (91 cells), p=0.989; SD 48 h: SD+Saline (39 cells) vs. SD+GA (90 cells), p=0.168; SD 72 h: SD+Saline (39 cells) vs. SD+GA (88 cells), p=0.931. (M) The fall time of Ca2+ transients in SD 24 h, SD 48 h, SD 72 h in SD and GA mice. Two-tailed unpaired t-test. SD 24 h: SD+Saline (40 cells) vs. SD+GA (91 cells), p=0.609; SD 48 h: SD+Saline (39 cells) vs. GA (90 cells), p=0.634; SD 72 h: SD+Saline (40 cells) vs. SD+GA (88 cells), p=0.648. n=6 SD mice, n=5 GA-treated mice. All data are shown as mean±SEM., *p<0.05, ns, not significant. (N) The mean amplitude of Ca2+ transients in recover 1 d, recover 2 d compare with baseline time 0 d in the SD+Saline group. Re 1 d vs. 0 d in SD+Saline mice. p=0.071; Re 2 d vs. 0 d in SD mice. p=0.15. (O) The mean amplitude of Ca2+ transients in SD 24 h, SD 48 h, SD 72 h, recover 1 d, recover 2 d, compare with 0 d baseline in SD+GA mice. 24 h: 0 d Baseline vs. SD+GA, p=0.977; 48 h: 0 d Baseline vs. SD+GA, p=0.562; 72 h: 0 d Baseline vs. SD+GA, p=0.871; Re 1 d: 0 d Baseline vs. SD+GA, p=0.819; Re 2 d: 0 d Baseline vs. SD+GA, p=0.393. n=42 cells in 6 SD+Saline mice, n=95 cells in 5 SD+GA mice. All data are shown as mean±SEM., ns, not significant, Two-tailed paired t-test.
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