Fig. 1. Lpc-EV treatment blocked GC-induced changes in the expression of neurotrophic factors, TrkB, Mkp1, Fkbp5, Hsp90a1, p53, and Mecp2 in HT22 cells. (A~C) Transcript levels of total Bdnf (tBdnf), Bdnf1, Bdnf3, Bdnf4, Nt3, Nt4/5, Ngf, and TrkB (A); Mkp1, Fkbp5, Hsp90a1, and p53 (B); Mecp2, Hdac2, and Sirt1 (C) in HT22 cells treated for 24 h with glucocorticoid (GC, corticosterone; 400 ng/ml) or GC plus Lpc-EV (10 μg/ml). (D~G) siRNA-mediated knockdown of Mecp2 (D). Transcript levels of tBdnf, Bdnf1, Bdnf3, Bdnf4, Nt3, Nt4/5, Ngf, and TrkB (E); Mkp1, Fkbp5, Hsp90a, and p53 (F); and Mecp2 (G) in HT22 cells treated for 24 h with GC (400 ng/ml), GC and Lpc-EV plus control-siRNA (siCON), and GC and Lpc-EV plus Mecp2-siRNA. Data are presented as the mean±SEM (n=8). *p<.05; **p<.01 (one-way ANOVA followed by Newman-Keuls post hoc test, and two-way ANOVA followed by Bonferroni post hoc test).
© Exp Neurobiol
