Fig. 5. Post-stress treatment with Lpc-EV reversed stress-induced changes in the expression of MeCP2, Sirt1 and neurotrophic factors in the hippocampus of CRST mice. (A) Experimental design. CRST mice were intraperitoneally (i.p.) treated with Lpc-EV for 14 days at a dose of 2 μg/100 μl/mouse. n=6~12 animals. (B, C) Expression levels of Mkp1, Fkbp5, and Hsp90a1 (B), and Mecp2 (C) in the hippocampus of CRST mice treated with Lpc-EV. Veh, vehicle (n=6~8 qPCR repeats). (D, E) Expression levels of Cbp, p300, Hdac1, Hdac2, Hdac3, Sirt1, and Sirt7 (D), and Kdm4a, Kdm4b, Suv39h1, and Setdb1 (E) in the hippocampus of CRST mice treated with Lpc-EV. Veh, vehicle (n=6~8 qPCR repeats). (F) Expression levels of total Bdnf (tBdnf), Bdnf1, Bdnf2, Bdnf3, Nt3, Nt4/5, Ngf, and TrkB in the hippocampus of mice treated with Lpc-EV. Veh, vehicle (n=6~8 qPCR repeats). Data are presented as the mean±SEM. *p<0.05; **p<0.01 (one-way ANOVA followed by Newman-Keuls post hoc test).
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