Fig. 3. Spinal astrocytic BDNF increases during the pain maintenance. (A) Experimental timeline for examining BDNF level in acute and chronic pain period. (B) Protein expression level of BDNF and TrkB in control, PCId7 and PCId28. (C) Normalization of BDNF expression in three groups. Unpaired-two-tailed-t-test, *p<0.05. (D) Immunohistochemistry representative images of GFAP and BNDF expression between ipsilateral and contralateral in control, PCId7 and PCId28. Scale bar: 10 µm. (E) Colocalization of BDNF in astrocytes positive among control, PCId7 and PCId28. Two-way ANOVA with Tukey’s multiple comparison test (**p=0.009, ***p<0.001, p>0.99).
© Exp Neurobiol
