Fig. 4. Reduced locomotion ability by ectopic expression of the C-terminal truncation mutation of hRAB40B. The locomotion ability of adult flies was quantified in a climbing assay. Progressive deterioration of motor performance was shown when the mutant hRAB40B was expressed ubiquitously (A, B, tub-GAL4) or under control of the pan-glial repo-GAL4 (B), but not with OK371 (A) or other neuron-specific GAL4 drivers. (C) The Drosophila Cul5 knockdown enhanced the locomotion defect with expression of the mutant hRAB40B, showing a genetic interaction of the defective motor phenotype with the CRL5-mediated proteolytic regulation (tested groups of each genotype, n=6; *p<0.05, **p<0.01, ***p<0.001).
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