Time-course alterations of tau phosphorylation in neuronal cell lines exposed at room temperature
| Epitope | Cell line | Sampling time (min) | |||||
|---|---|---|---|---|---|---|---|
| 0.5 | 1 | 2 | 5 | 10 | 15 | ||
| AT270 | SH3R | -3 | +7 | +34 |
+69 |
+95 |
+76 |
| N2a | +52 |
+65 |
+75 |
+90 |
+80 |
+52 |
|
| pSer199 | SH3R | +38 |
+75 |
+75 |
+58 | +39 | +26 |
| N2a | -10 | -0.1 | +24 |
+30 |
+64 |
+56 |
|
| CP13 | SH3R | -12 | -27 |
-19 | -23 | +12 | +114 |
| N2a | +18 | +94 |
+72 |
+75 |
+93 |
+45 |
|
| pThr205 | SH3R | -19 |
-21 |
-8 | +1 | +20 | +43 |
| N2a | +17 | +36 |
+42 |
+62 |
+118 |
+45 |
|
| pSer409 | SH3R | +16 | +11 | +10 | +15 | +45 |
+67 |
| N2a | +58 |
+28 |
+29 |
+70 |
+92 |
+51 |
|
| AT100 | SH3R | +15 | +32 |
+36 |
+50 |
+77 |
+114 |
| MC6 | SH3R | +23 |
+23 |
+5 | +23 | +9 | +78 |
| pSer262 | SH3R | +49 |
+63 |
+55 |
+78 |
+46 |
+82 |
| PHF1 | SH3R | -20 |
-22 |
-30 |
-39 |
-35 |
+86 |
| pSer422 | SH3R | +20 | +32 |
+78 |
+103 |
+136 |
+151 |
Tau phosphorylation artifacts occurring over minutes was observed in SH3R and N2a cells following their removal from incubation and exposure to room temperature. The values indicate the percentage of phosphorylation changes relative to the baseline control condition (t=0). Significant increases in tau phosphorylation are denoted by a light-yellow background, while significant tau dephosphorylation is represented with a light-blue background.
*p<0.05, **p<0.01 and ***p<0.001 vs. control condition.