Fig. 2. . Influence of room temperature exposure on tau-related enzymes and chaperones. The expression level of (A) ratio of inactive pGSK3β(pSer9)/GSK3β, (B) ratio of pJNK/JNK, (C) ratio of inactive demethylated PP2AC/PP2AC, (D) HSP70 and (E) HSP90 were determined by Western blot in cells harvested immediately (t=0, control) or at t=0.5; 1; 2; 5; 10; 15 min (time spent at RT). HSP70 and HSP90 were normalized over actin levels. For each condition, a representative band is presented above graphs. Data are presented as mean±SEM and expressed as percentage of control condition (t=0). 1-way ANOVAs followed by Dunnett’s post-test were performed: RT pGSK3β(pSer9): F6,46=9.22 (p<0.0001); pJNK: F6,26=18.02 (p<0.05); demethylated PP2AC: F6,51=5.712 (p<0.0001); HSP70: F6,45=2.48 (p<0.0001); HSP90: F6,45=2.47 (p<0.05); ice pGSK3β(pSer9): F6,45=1.23 (ns). *p<0.05, **p<0.01 and ***p<0.001 vs. control condition. A second 1-way ANOVA followed by Dunnett’s post-test was performed only on the first timings (from t=0 to t=5 min): pGSK3β(pSer9): F4,35=4.13 (p<0.01); pJNK: F4,17=10.59 (p<0.001); demethylated PP2AC: F4,39=6.58 (p<0.001); HSP70: F4,33=1.37 (ns); HSP90: F4,33=2.57 (ns). +p<0.05, ++p<0.01 and +++p<0.001 vs. control condition.
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