Exp Neurobiol 2012; 21(3): 83-93
Published online September 30, 2012
© The Korean Society for Brain and Neural Sciences
Seung Baek Han1,2, Hyukmin Kim1,2, Andrew Skuba1,2, Alan Tessler1, Toby Ferguson1,3 and Young-Jin Son1,2*
1Shriners Hospitals Pediatric Research Center and Center for Neural Repair and Rehabilitation, 2Department of Anatomy and Cell Biology, 3Department of Neurology, Temple University School of Medicine, Philadelphia, PA 19140, USA
Correspondence to: *To whom correspondence should be addressed.
TEL: 1-215-991-8274, 1-215-926-9354, FAX: 1-215-843-9082
Injured primary sensory axons fail to regenerate into the spinal cord, leading to chronic pain and permanent sensory loss. Re-entry is prevented at the dorsal root entry zone (DREZ), the CNS-PNS interface. Why axons stop or turn around at the DREZ has generally been attributed to growth-repellent molecules associated with astrocytes and oligodendrocytes/myelin. The available evidence challenges the contention that these inhibitory molecules are the critical determinant of regeneration failure. Recent imaging studies that directly monitored axons arriving at the DREZ in living animals raise the intriguing possibility that axons stop primarily because they are stabilized by forming presynaptic terminals on non-neuronal cells that are neither astrocytes nor oligodendrocytes. These observations revitalized the idea raised many years ago but virtually forgotten, that axons stop by forming synapses at the DREZ.
Keywords: dorsal root entry zone, sensory nerve regeneration, NG2 glia, oligodendrocyte precursor cells, in vivo imaging, astrocytes
The cell bodies of dorsal root ganglion (DRG) neurons, which relay sensory information into the spinal cord, are located in peripheral ganglia. They emit one process that bifurcates into a peripheral axon branch and another that projects centrally into the spinal cord within the dorsal root. Dorsal roots, the central branches of DRG neurons, mount a far weaker regenerative response than other peripheral nerves [1-4]. Unlike motor nerves and peripheral branches of DRGs, DR axons often fail to regenerate across a transection site .
After DR crush, a less severe injury that does not interrupt root continuity, axons regenerate along the root but more slowly than other peripheral nerves. Their regeneration ceases at the dorsal root entry zone (DREZ), the transitional zone between the CNS and PNS. As first shown in the drawing of Ramòn y Cajal (Fig. 1), DR axons turn around and grow back to the PNS (arrow), or stop at (arrowheads) the DREZ. What prevents the regeneration of DR axons across the DREZ remains unclear. Inhibition by CNS glia such as astrocytes and oligodendrocytes makes an important contribution to regenerative failure at the DREZ, but the decisive factor(s) and their mechanisms of action are unknown [6-12]. Also unknown is whether the growth inhibitory activities at the DREZ are the same or different from those elsewhere within the CNS [6, 13, 14].
Although interventions that enhance the regeneration capacity of DR axons by means of neurotrophic factors or that neutralize astrocyte- or myelin debris-associated growth-inhibitors have been partially effective [7, 14-23], no strategies have promoted regeneration by all or most DRG neuron subtypes. A glial cell line-derived neurotrophic factor (GDNF), artemin, induces topographically specific regeneration of most DR axons in rodents [24, 25] but, like other neurotrophic factors, severe side effects preclude its use in clinic trials. The need to develop new treatments is urgent.
Dorsal root injuries, which include brachial plexus, lumbosacral plexus and cauda equina injuries, result in permanent loss of primary afferent terminals in the spinal cord. These injuries have profound effects on the spinal cord and evoke chronic, often agonizing, pain and permanent loss of sensation. Brachial plexus injury (BPI), the most common form of DR injury, results from high-energy traction injuries in which the head and neck are forced away from the shoulder. Obstetrical BPI is the most common etiology of a plegic arm in infants, occurring in ~3 per 1,000 live births. In adults, BPI occurs most commonly in motor vehicle accidents, particularly motorcycles, contact sports and falls. Overall, DR injuries are 10~20 times more common than spinal cord injury (SCI) [13, 26-28]. There is an urgent unmet clinical need for effective therapies that can reduce the extent of the initial injury or, at a later stage, enhance repair. The need for effective treatment is increasing due to higher survival rates following severe traumatic injuries and the increasing number of elderly individuals susceptible to these injuries because of falls.
Clinical treatment of brachial plexus injury is often surgical. In children, damaged peripheral components of the plexus may be repaired with donor nerve (usually the patient's own sural nerve) or other graft material, including processed cadaver nerve or tubes containing extracellular matrix [29, 30]. In adults, the distance that regenerating nerves must grow is often too lengthy for effective grafting. Nerve anastomosis to a nearby denervated nerve and muscle may be useful in both children and adults [31, 32]. However, it must be emphasized that recovery after peripheral injury is generally incomplete even with these treatments and that there is no effective therapy for dorsal root avulsions.
At the DREZ, the glial ensheathment of the axons changes abruptly from Schwann cells in the dorsal root to astrocytes and oligodendrocytes in the spinal cord. Following DR injury, on the PNS side, macrophages rapidly phagocytose myelin and degenerating axons , while Schwann cells become activated/dedifferentiated and occupy axon-free endoneurial tubes. By contrast, on the CNS side, astrocytes rapidly undergo reactive changes, which include proliferation and extension of their processes further distally into DR, between the endoneurial tubes (i.e., astrogliosis) [34, 35]. Following DR crush, injured axons regenerate past the crush site, but turn around or stop at the DREZ. DR injury evokes changes similar to those induced by direct spinal cord injury (SCI), without the formation of a dense astrocytic glial scar. Nevertheless, the axotomized DREZ prevents regeneration surprisingly efficiently: Whereas peripheral conditioning lesions promote intraspinal regeneration of their central axons in the dorsal columns [36, 37], the same axons fail to regenerate through the DREZ, as confirmed in our
What prevents axons from regenerating across the DREZ? The prevailing view in the field is that regeneration is prevented at the DREZ primarily by growth-inhibiting activities associated with reactive astrocytes and/or degenerating oligodendrocytes. Because axons contact astrocytes when they have stopped regeneration at the DREZ [40-42], reactive astrocytes are thought to form the primary regenerative barrier. Consistent with this notion, axons grow through the DREZ that has been depleted of astrocytes by X-irradiation , and, in general, (despite exceptions [6, 44]), reactive astrocytes inhibit neurite outgrowth [8, 45] by producing chondroitin sulfate proteoglycans (CSPGs) that collapse or repel neurite outgrowth [46, 47]. Members of the CSPG family of extracellular matrix molecules include neuroglycan 2 (NG2), aggrecan, brevican, neurocan, vesican and phosphacan . These CSPGs are expressed at the DREZ both during development and after dorsal root injury .
The differential expression and contribution of individual members of the CSPG family have also been studied. NG2, the most important component, is a major inhibitory proteoglycan for sensory axons . NG2 is expressed by oligodendrocyte progenitor cells, which react rapidly following CNS injury, and by some reactive astrocytes. Virus-mediated knockdown or antibody blocking of NG2 promotes intraspinal sensory axon regeneration . Recently, a transmembrane protein tyrosine phosphatase, PTPσ, was identified as a high affinity receptor of CSPG that mediates its inhibitory effect [51, 52]. Disruption of the PTPσ gene reduced inhibition by CSPG.
Several lines of evidence argue that astrocytes and CSPGs are not the critical determinant of regeneration failure. For example, the same CSPGs are expressed abundantly in tissue that supports regeneration (dorsal roots) and in tissue in which regeneration fails (DREZ and spinal cord) . The inhibitory properties of CSPGs are primarily due to glycosaminoglycan (GAG) side chains; enzymatic removal of GAG chains by chondroitinase ABC (ChABC) promotes intraspinal axon regeneration [46, 54]. However, pharmacological degradation of CSPGs by chondroitinase ABC or Phosphatidylinositol-specific phospholipase C (Pi-PLC), which enhances regeneration in the damaged spinal cord , does not promote regeneration across the DREZ [23; but see Cafferty et al., 2007]. How astrocytes prevent regeneration, if they indeed play a crucial role in the regeneration failure, remains uncertain.
Oligodendrocyte/myelin-derived inhibitors, such as Nogo-A, myelin-associated glycoprotein (MAG) and oligodendrocyte-myelin glycoprotein (Omgp), may also contribute to regeneration failure at the DREZ . These molecules, except MAG, are distributed exclusively in CNS myelin synthesized by oligodendrocytes and are not found in PNS myelin synthesized by Schwann cells. All three of these myelin inhibitors bind to the glycosylphosphatidylinositol-anchored Nogo-66 receptor (NgR1), which is expressed by many CNS neurons [56, 57]. Other receptors, including NgR2 and the paired immunoglobulin-like receptor B (PirB), have also been implicated in mediating the inhibitory growth signaling [58, 59].
Intrathecal application of the soluble Nogo-receptor peptide fragment of the NgR (sNgR) after DR crush elicited regeneration of myelinated, but not unmyelinated, sensory axons . This result supports the idea that myelin-associated molecules contribute to regeneration failure. It is important to note, however, that myelin-associated molecules are eventually, although slowly, cleared, together with degenerating oligodendrocytes [35, 38, 40]. Thus, their actions are exerted only transiently during the initial phase of inhibition. In addition, it is noteworthy that axons can regenerate along degenerating white matter [60, 61], and that simultaneous elimination of multiple inhibitory molecules does not promote intraspinal regeneration [62, but see, 63]. Whether regeneration is promoted at the DREZ in these triple knockout mice has yet to be determined.
Lastly, but perhaps most importantly, myelin-derived inhibitors and CSPGs act as repellent cues that cause only brief growth cone collapse or retraction [10, 64, 65]. Furthermore, DRG axons can grow despite growth cone collapse [66-68]. Moreover, unlike
One provocative idea raised many years ago that has received little subsequent attention is that astrocytes induce DR axons to form synapses that immobilize them at DREZ. Carlstedt first made this proposal based on his observation of structures at DREZ that he termed synaptoids , because of their interesting resemblance to nerve terminals. Other investigators have observed similar nerve terminal-like structures [38,69], and Liuzzi and Lasek also speculated that astrocytes provide a physiological 'stop signal' that prevents regeneration of DR axons at the DREZ . As shown in Fig. 2, these axonal profiles resemble nerve terminals because mitochondria and vesicles are copious whereas microtubules and neurofilaments are sparse or absent. These 'synaptoid' profiles lack some characteristic features of pre- and postsynaptic differentiation, however, and mitochondria and vesicles are also abundant even in non-synaptic dystrophic endings . In addition, none of the early studies provided direct evidence that these synapse-like profiles belong to sensory axon growth tips, rather than to other neuronal elements such as the chronically remodeled dendrites of dorsal horn neurons. These studies also did not determine whether axon endings became 'nerve terminal-like' as they entered the DREZ or whether the nerve terminal-like appearance developed only after chronic remodeling.
Nonetheless, it is notable that these nerve endings clearly differ from the retraction bulbs, originally described by Cajal (Fig. 1), formed by severed dorsal column axons and from the dystrophic endings formed in an
Until recently biologic processes could only be analyzed using static images obtained after death from animals euthanized at multiple time points. The temporal and spatial resolution of these analyses was limited because dynamic changes had to be deduced from comparisons of static images. Accordingly, prior studies based on conventional static analyses provided evidence that is often conflicting or inconclusive. Dynamic cellular processes, including axon regeneration and the interactions between axons and their environment that determine the success or failure of regeneration, are best studied with techniques that capture real-time events with multiple observations of each living animal.
Our ability to monitor neurons serially
Axon sparing has been responsible for conflicting or inclusive observations in studies of spinal cord or root injuries . We consider the power to distinguish between spared and regenerating axons an important asset of
If growth-inhibitory molecules prevent regeneration of DR axons at DREZ, in agreement with, the predominant view in the field, then one would predict dynamic responses by DR axons at DREZ. After encountering CSPGs and myelin-associated inhibitors that are produced by reactive astrocytes and degenerating oligodendrocytes in the spinal cord and cause growth cone collapse, axons would be expected to regenerate back into the PNS, where growth-promoting Schwann cells are present, or to continue to try to grow within the DREZ and then to degenerate. Surprisingly, our
Light microscopic and ultrastructural analyses targeted specifically to axon tips monitored
Our findings conflict with the prevailing view that axons stop at the DREZ by forming swollen dystrophic endings . We have found that surprisingly few stalled axons terminate in large swollen tips and that most end as slender tips that persist . Notably, almost all axons, including those with large swollen endings, display small varicosities along their shafts that are intensely immunolabeled with synapse markers . Our recent EM study confirmed that the large swollen endings are indeed dystrophic and lack synaptic characteristics. Importantly, however, these axon tips are completely surrounded by acellular collagenous fibers (Skuba et al., unpublished data), rather than by non-neuronal cellular elements, and they are located superficially where collagenous scars have formed adjacent to dura. Together, our findings show that most, if not all, DR axons become arrested as they enter the CNS territory of the DREZ by forming presynaptic terminals on non-neuronal cellular elements. These observations are also in line with the provocative idea that astrocytes induce DR axons to form 'synaptoids' that cause their arrest at the DREZ [38, 40, 69].
The identity of the postsynaptic cells has yet to be determined. The absence of postsynaptic densities excluded the possibility that these profiles are axon-axon synapses , and the cells lack the intermediate filaments that are abundant in astrocytes . Some evidence suggests that they may be NG2 cells forming synapses with neurons. NG2 cells are proteoglycan NG2 (nerve/glial antigen 2)-expressing oligodendrocyte progenitors which constitute a fourth major glial cell in the mammalian CNS, distinct from astrocytes, oligodendrocytes and microglia [86-93]. They are widely distributed in both gray and white matter, and have a complex stellate morphology with highly branched fine processes [86, 94-97]. They have also been reported to form a cellular net in the CNS territory of the DREZ [98, 99], proximal to the astrocyte-Schwann cell border, where our
Several caveats concerning the imaging studies, however, make our interpretation tentative and justify a more thorough investigation. A particular concern is that the limited spatiotemporal resolution of our axon growth tip imaging might have caused us to overlook subtle local motility. In addition, because we imaged only a few large-diameter axons in a small number of mice, we have little information about possible variability in the responses of the heterogeneous populations of DRG neurons. Imaging more DR axons of all different types at higher spatiotemporal resolution will therefore be necessary. Direct demonstration
Both intrinsic and extrinsic factors, particularly growth-inhibitory molecules associated with astrocytes and oligodendrocytes, are thought to contribute to regeneration failure at the DREZ. Several observations previously reported in the literature, however, make it unlikely that these factors are the critical determinant(s) of the regeneration failure: 1) Even after myelin-associated inhibitory cues have disappeared due to oligodendrocyte degeneration, the DREZ continues to prevent regeneration; 2) CSPGs associated with astrocytes and myelin-associated inhibitory cues collapse growth cones transiently, but do not immobilize or chronically stabilize growth cones; 3) Although central processes of DRG neurons in the DC and DR derive from the same neurons and encounter the same inhibitory cues, conditioning lesions enhance regeneration of DC axons [36, 102] but not DR axon regeneration across DREZ ; 4) CSPGs are expressed abundantly in the regenerating DR  and pharmacological degradation of CSPGs by ChABC does not promote regeneration across DREZ . The first application of