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Exp Neurobiol 2014; 23(1): 53-64
Published online March 30, 2014
© The Korean Society for Brain and Neural Sciences
Jaekwang Lee1,3, Oleg V Favorov1, Mark Tommerdahl1, C. Justin Lee3,4* and Barry L. Whitsel1,2*
1Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA, 2Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA, 3WCI Center for Functional Connectomics, Institute of Science and Technology (KIST), Seoul, Korea, 4KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea
Correspondence to: *To whom correspondence should be addressed.
C. Justin Lee
TEL: 82-2-958-6940, FAX: 82-2-958-6937
Barry L. Whitsel
TEL: 1-919-966-1291, FAX: 1-919-966-6927
It has been reported that long-term enhancement of superficial dorsal horn (DHs) excitatory synaptic transmission underlies central sensitization, secondary hyperalgesia, and persistent pain. We tested whether impaired clearance of K+ and glutamate by glia in DHs may contribute to initiation and maintenance of the CNS pain circuit and sensorimotor abnormalities. Transient exposure of the spinal cord slice to fluorocitrate (FC) is shown to be accompanied by a protracted
Keywords: dorsal horn, astrocytes, glia-neuron interactions, central sensitization, hyperalgesia, persistent pain
Direct application to the spinal cord of a variety of substances (e.g., excitatory neurotransmitters such as substance P or NMDA receptor agonists) or low-frequency nociceptor afferent input can produce a long-term enhancement of excitatory synaptic transmission between C-fiber afferents and neurons in the DHs [1, 2, 3]. Clearly, therefore, establishment of the long-term enhancement of DHs excitatory synaptic transmission that underlies central sensitization, secondary hyperalgesia, and persistent pain does not, as previously believed, require a sustained preceding period of high-frequency nociceptor afferent activation.
The experiments described in this paper were motivated by the intriguing and clinically relevant possibility that a long-term enhancement of DHs excitatory synaptic transmission not only can occur in the absence of a preceding period of conditioning afferent drive (as described above), but can arise subsequent to an impairment of DHs glial energy metabolism. Observations reported in previous studies are fully consistent with the idea that impairment of the ability of astrocytes to regulate extracellular K+ and glutamate levels alters excitatory neurotransmission at multiple levels of the CNS. As examples: (1) the secondary hyperalgesia that follows intradermal injection of 5% formalin is accompanied by an impaired ability of astrocytes to regulate [K+]o and [glutamate]o in the region of the DHs that receives its input from the injected skin site [4, 5], (2) even a brief (40 sec) increase in extracellular K+ induces LTP in the hippocampal slice ; (3) hippocampal LTP is substantially reduced in animals in which astrocytes are rendered unable (via a selective genetic manipulation) to release neurotransmitters ; (4) d-serine released from astrocytes is critical for the induction of LTP in hippocampal slices ; (4) the slow astrocytic depolarization that accompanies hippocampal LTP is due to astrocyte uptake of K+ and supports neuronal LTP by reducing the efficacy of astrocytic glutamate transporters and inducing astrocyte release of a variety of signaling molecules (for review see ); and, finally, (5) intracerebral injection of fluorocitrate (a reversible, selective inhibitor of glial metabolism, [5, 10]) elevates [K+]o, increases cortical neuron excitability, and induces focal epileptiform discharge .
The goals of this study were to: (1) investigate the possibility that transient impairment of spinal cord glial energy metabolism can, in the absence of a preceding period of conditioning afferent drive, initiate the long-term enhancement of DHs excitatory synaptic transmission widely regarded to underlie central sensitization, secondary hyperalgesia, and persistent pain [3, 12]; and, (2) acquire information bearing directly on the validity of the recent proposal that impaired astrocytic regulation of [K+]o can lead to conversion of the effect of GABA on DHs neurons from inhibition to excitation [4, 13, 14].
Transverse slices of the lumbosacral cord (400~800 µm thickness) were cut using an oscillating tissue slicer (OTS-4000, Electron Microscopy Sciences, Vibratome 3000) and placed in a reservoir containing ACSF warmed (30℃) and oxygenated (using a 95% O2 and 5% CO2 gas mix). Slices remained in the reservoir (never less than 1 hr) until transferred by pipette to a submerged position in a recording chamber perfused with warmed (28~30.8℃) and oxygenated ACSF (perfusion rate 2~3 ml/min). Composition (in mM) of the ACSF delivered to the slice prior to treatment with a drug or drug combination was 124 NaCl, 3.0 KCl, 2.5 CaCl2, 25 NaHCO3, 1 MgSO4, 1.25 NaH2PO4, and 10 glucose. A total of 22 slices were studied.
The procedure reported by Paulsen et al. (1987) was used to prepare dl-fluorocitric acid (FC; Sigma). The barium salt of FC was dissolved in 0.1 M HCl; 0.1 M Na2SO4 was added to precipitate barium, and the solution centrifuged at 800~1,000 g for 5~10 min after buffering with 0.1 M NaPO4-. The supernatant containing FC then was transferred and added to ACSF to achieve a stock FC concentration of 1 mM. For the experiment the stock solution of 1 mM FC in ACSF was diluted to the desired concentration by adding additional ACSF or, when required, d-2-amino-5-phosphonopentanoic acid (D-APV; Sigma) and/or bicuculline (Bic; Sigma) were added to achieve the desired concentrations of FC and drug or FC + drug-combination in ACSF. After warming and oxygenation drug-containing ACSF was delivered to the submerged slice via the chamber perfusion system. In other experiments the solution used to perfuse the slice was switched to ACSF containing a less-than-normal concentration of K+ (i.e., 0.0, 1.0, or 2.0 mM rather than 3.0 mM; accomplished by equimolar substitution of NaCl for KCl).
The slice was transilluminated and images obtained at ×2 or ×4 magnification using an inverted microscope (Diaphot 200, Nikon) and a cooled, slow-scan CCD camera (Photometrics Inc.). An optical intrinsic signal (OISDR) was evoked within the DHs by application of a repetitive constant-current stimulus to the dorsal entry zone using a glass insulated, 50 µm diameter, metal bipolar stimulating electrode connected to an isolation unit, and programmable pulse generator (Master-8, AMPI). Stimulation parameters were: pulse duration - 0.2 ms; intensity - 2~4× the threshold current for evoking an optical response; train duration - 1.0 s; frequency - 20 Hz; intertrain interval - 5 min. Each image included all of the dorsal horn on the same (ipsilateral) side as the stimulated dorsal root, much of the adjacent ventral horn, and a large portion of the bounding white matter. 30 images were obtained in association with each repetitive dorsal root stimulus (a "trial"). The 1st and 2nd images ("reference" images) in each trial were obtained at 1,000 ms and at 500 ms, respectively, prior to stimulus onset; 2 "poststimulus onset" images were acquired during delivery of the repetitive stimulus, and the remaining 26 images after stimulus termination (image acquisition rate=2/s). Trial duration was 9 s.
An average (across-frame) difference image was generated from the optical response to each repetitive dorsal root stimulus by (1) subtracting the reference image obtained at 500 ms before stimulus onset from each image obtained in the same trial between 2.0~7.5 s after stimulus onset (images 6~25; total of 20), and (2) at each pixel location by dividing the sum of the differences between the post-stimulus and reference images (same-trial) by the number of frames. An intensity value was calculated for each pixel in a difference image using the formula Σ(
Single-pulse (0.2 ms) constant-current stimuli were applied to the dorsal root using a suction electrode, isolation unit, and programmable pulse generator (Master 8, AMPI). The population postsynaptic potential evoked by the dorsal root stimulus (P-PSPDR) was recorded with a 1~2 mM NaCl-containing micropipette (1.2 mm OD). All recordings of the P-PSPDR were obtained from a locus in the substantia gelatinosa (SG) medial to the dorsal root entry zone - at this site the OISDR is maximal and the recorded field potential consists of both short- and longer-latency responses to single-pulse stimulation of the dorsal root [15, 16, 17]. P-PSPDRs were evoked using stimulus intensities 2~4× the minimum ("threshold"; typically 50~80 µA) current required to elicit a P-PSPDR. Such currents activate both large- (Aβ) and small-diameter (Aδ and C) afferents in the dorsal root [17, 18, 19, 20, 21, 22]. Recordings were filtered (30~300 Hz) and sampled at 50 kHz using pClamp 7.0 (Axon Instruments). Individual field potential recordings were quantified by measuring the baseline-to-peak amplitude between 0~20 ms after stimulus onset; time course of the effect of each experimental manipulation was determined by plotting average evoked potential amplitude vs. time.
In experiments of this type the slice was transferred from the reservoir to the recording chamber of a fixed-stage upright microscope (Olympus BX51WI) and visualized directly via the microscope's optics, or indirectly via a high resolution CCD camera system (CCD-100, Dage-MTI, Inc) that received the output of a CCD camera attached to the microscope's video port. Whole cell patch-clamp recordings were obtained from lamina II neurons using borosilicate glass pipettes (resistance 4~6 MOhm) pulled on a 2-stage vertical pipette puller (Narishige PC-10), and filled with a solution with the following composition (in mM): 120.0 K-gluconate, 20.0 KCl, 2.0 NaCl2, 20.0 HEPES, 0.5 EGTA, 10.0 glucose, 2.0 Na-ATP, 0.5 Na-GTP; pH adjusted to 7.3 with KOH. Patch recordings were obtained under the same slice conditions (temperature, perfusion rate, etc.) used to obtain OIS imaging and field potentials observations. Voltage-clamp and current-clamp recordings were carried out with a MultiClamp 700B amplifier, using pClamp9 acquisition software (Molecular Devices, Union City, CA, USA). Signals were filtered at 50~500 Hz, sampled at 5 kHz for identification of cell type and at 50 Hz for the GABA application part of the experiment. Data were analyzed and plotted using Origin 7.0. No correction for liquid junction potential was made. Cells with resting potentials greater than -60 mV or less than -80 mV were regarded as "abnormal" and were not studied further.
Once a neuron was accepted for study the tip of a glass pipette filled with 1 mM GABA was placed (using a 3-axis micropositioner; NMN-25, Narishige Group Inc., Tokyo, Japan) at a location within 50~100 µm of the soma. The GABA equilibrium potential (EGABA) was determined by measuring the transmembrane current evoked by a temporally controlled, local application of GABA (a 300 msec "puff " delivered at 3~5 psi via the pipette filled with 1 mM GABA; using Picospritzer II, Parker Hannifin Corp.) at holding potentials ranging between -100 mV and -20 mV (step size=20 mV), both before and after exposure of the slice to FC. Each GABA puff applied during the study of a neuron was delivered at the same location in the DHs, and at the same pressure and duration.
In each of 3 slices the OISDR evoked in the ipsilateral DHs by electrical stimulation of the dorsal root was recorded at regular intervals (i) before ("Control"), (ii) throughout a 20 min exposure to ACSF containing 50 µM FC ("FC"), and (iii) during the 60 min period following the restoration of perfusion with drug-free ACSF ("Washout"). Labels in the top left image in Fig. 1A (grayscale) identify the position of the DHs, the location of the tip of the stimulating electrode (E - in the part of the dorsal root entry zone that overlies the medial half of the DHs), and the structures which border the DHs on the left side of the slice (e.g., DC - dorsal column; LC - lateral column; VH - ventral horn). The colour-coded average difference images in Fig. 1A (i.e., average prestimulus - poststimulus images) show that not only did it decrease in both intensity (ΔT/T) and spatial extent during the 20 min exposure to 50 µM FC (FC
The superimposed field potential recordings (each trace is the average response to 10 stimulus trials) in Fig. 1C illustrate the effect of FC on the population postsynaptic potential (P-PSPDR) evoked in the ipsilateral DHs by a single-pulse constant-current stimulus to the attached dorsal root. Note that although for this exemplary slice the P-PSPDR recorded during the exposure to FC (orange trace - FC) is slightly smaller than the potential recorded prior to the exposure to FC (black trace - Control), the P-PSPDR recorded after restoration of the perfusion solution to drug-free normal ACSF (red trace - Washout) is substantially larger than the P-PSPDR recorded prior to the exposure to FC.
Fig. 1D shows the average across-slice (n=6) time course of the effect of FC on the P-PSPDR (plot with filled circles). In each of 6 slices studied in this way exposure to FC was followed (during the initial 20~35 min of the 60 min washout period) by a substantial increase in P-PSPDR amplitude (the peak increase in the P-PSPDR during washout ranged between 140~168%; mean=153%; n=6). Over the last 30~35 min of the washout period P-PSPDR magnitude declined progressively, but in no slice did P-PSPDR amplitude return to values measured prior to FC exposure (P-PSPDR amplitude at the end of the 60 min washout period was 115~132% of Control; mean=124%; n=6).
Similar to the findings reported in previous studies of the long-term effects of repetitive afferent drive on glutaminergic neurotransmission at a variety of sites in the CNS (e.g., spinal cord dorsal horn - ; hippocampus - [23, 24, 25]; neocortex - [26, 27]), LTPFC apparently requires NMDA receptor activation because no facilitation of DHs excitatory neurotransmission occurred when APV (at a concentration that achieves a nearly complete block of NMDA receptors; 50 µM; plot with open circles at Fig. 1D; n = 5) was present in the perfusion solution during the time the slice was exposed to FC. In contrast, FC's suppression of the OISDR apparently does not require NMDA receptor activation because exposure to APV+FC (plot with open circles at Fig. 1B) failed to significantly alter either the magnitude or time course of the reduction of the OISDR that followed exposure to FC. Consistent with the demonstration that magnitude of the OISDR depends on glutamatergic neurotransmission [4, 21], APV application alone led to a modest suppression of the OISDR (plot with gray squares at Fig. 1B) that reversed and returned to control levels 20~25 min after the perfusion solution was restored to drug-free, normal ACSF.
The possibility that excess local accumulation of K+ in the DHs might, at least in part, be responsible for FC's long-term alteration of DHs excitatory neurotransmission was evaluated by varying (from one slice to the next) the [K+]o in the perfusion solution during the exposure to FC. The plots of P-PSPDR magnitude vs. time in Fig. 2A show that the effect of the exposure to FC modified strikingly and systematically as [K+]o in the perfusion solution (and, therefore, in the DHs) was varied over the range 3.0~0.0 mM. Reduction of K+ in the ACSF by 1 mM (i.e., to 2 mM; plot with open squares in Fig. 2A) eliminated the long-term enhancement of DHs neurotransmission detected when FC was provided to the slice in normal ACSF. Moreover, when [K+] in the perfusate was 1 mM or less, exposure to FC was followed by a long-term depression of the P-PSPDR (LTDFC; plots with filled and open circles). Statistical analysis of the data obtained from each slice and under each condition at 30 min after exposure to FC revealed that the [K+]o dependency of FC's effect on the magnitude of the DHs response to small-diameter afferent drive is linear and statistically significant (Fig. 2B; p=0.034; P-PSPDR=-65.4+36.8×[K+]o; X-intercept=1.8 mM [K+]o).
A third and final series of field potential recording experiments was carried out to determine if manipulation of [K+]o in the perfusion solution would modify the long-term potentiation of DHs excitatory neurotransmission that develops in normal ACSF (containing 3.0 mM [K+]o ) following an exposure to FC. The result obtained from every slice (n = 4) studied in this way was consistent and unambiguous (Fig. 3) - that is, at the time at which the effect of FC on the P-PSPDR was maximal (at 30 min after the exposure to FC) switch of the perfusion solution to ACSF containing zero K+ was accompanied by a rapid decrease of the P-PSPDR to pre-FC (control) values. Clearly, therefore, DHs excitatory neurotransmission remained exquisitely sensitive to [K+]o after the exposure to FC.
The superimposed field potential recordings at the top left of Fig. 4A illustrate a prominent characteristic of field potential recordings obtained from the medial DHs (e.g., see , also ) - that is, high-frequency and rhythmic postsynaptic neuronal activation is apparent in the response to dorsal root stimulation (gray trace for the 3 conditions - control, after FC, and zero K+), as well as in across-trial (n = 10) averages of the DHs response obtained under the same conditions (black traces). Inspection of the single-trial and average across-trial responses recorded under the 3 conditions shows that: (1) exposure to FC not only is followed by a large increase in the magnitude of the average across-trial DHs field potential (LTPFC), but also by a prominent increase in the synchronous, high-frequency DHs activation observable in the response to each stimulus; and (2) both the LTPFC and the increased high-frequency activation induced by FC are substantially reduced when the solution perfusing the slice is switched to ACSF containing zero K+.
FFT analysis of the single-trial responses (histograms on Fig. 4B) quantitatively confirmed that: (1) FC exposure was followed by a prominent increase of the synchronized, high-frequency component of the DHs response to the dorsal root stimulus; and (2) perfusion of the slice with ACSF containing zero K+ eliminated the enhancement of the high-frequency component that occurred following the exposure to FC. The pseudocolour 3-D plot (upper part) and surface projection map (lower part) in Fig. 4C illustrate (for the same slice that yielded the responses in Fig. 4A) the characteristic temporal attributes of the high-frequency DHs activation evoked by a dorsal root stimulus following exposure to FC - i.e., multiple peaks in the DHs field potential; neighboring peaks separated by a 5~8 msec interval during which voltage was at or near-baseline.
The recently published demonstration that GABAA receptor block reverses the injury-induced sensitization of nociceptor-specific neurons in the DHs of intact animals , together with the proposal  that GABA becomes a postsynaptic excitatory neurotransmitter in the DHs following peripheral nerve injury motivated us to evaluate the impact, if any, of GABAA receptor block on the LTP induced by FC. Panel A in Fig. 5 shows field potential recordings (P-PSPDRs) obtained from the ipsilateral DHs of an exemplary subject before ("Control"), 30 min after bath application of FC ("after FC"; revealing a prominent LTPFC) and, finally, 30 min after exposure of the same slice to ACSF containing 20 µM bicuculline ("after Bic"; revealing that after exposure to Bic the LTP induced by FC was no longer evident). The plot in panel B shows the time course of the effect of Bic on the LTP induced by exposure to FC (average of data obtained from 4 slices studied in the same way). These findings reveal that LTPFC depends on GABAA receptor-mediated neurotransmission because, like its effect on the dorsal horn sensitization induced by application of algesic chemical to the receptive field , the GABAA receptor block achieved by Bic antagonizes the LTP induced by exposure to FC.
The current vs. time traces in Fig. 5B show the transmembrane currents evoked at different holding potentials in a representative DHs neuron in response to the a brief increase in the local concentration of GABA ("puff"), both before ("Control", top left) and after (FC; top right) a 20 min exposure of the slice to 50 µM FC. The I-V curves shown as insets below the traces in Fig. 5B demonstrate that while the relationship between GABA-evoked peak current and membrane potential is essentially linear both before and after FC, the X-intercept of the best-fitting linear regression line (indicating the equilibrium potential for the ionic currents triggered by GABA - EGABA) for the observations obtained after the exposure to FC (approx. -50 mV) is substantially more positive than the X-intercept for the data obtained prior to FC (approx. -75 mV).
The lower left and right panels of Fig. 5B show I-V curves obtained by averaging the data obtained from 5 neurons studied using the above-described protocol (enabling measurement of peak transmembrane current evoked in each neuron before and after exposure to FC by GABA application at holding potentials stepped between -20 and -100 mV). As was the case for the neuron that provided the data in Fig. 5B (upper), both the acrossneuron (n=5) before-FC (on the left; Fig. 5B) and after-FC (on the right; Fig. 5B) I-V curves are well-fit by linear regression lines (p<.002), and the X-intercept (indicating EGABA) for the post-FC observations is substantially and statistically different (p<.001) from the X-intercept for the pre-FC observations (exposure to FC is associated with a substantial depolarizing alteration - approximately 31 mV - of EGABA; i.e, EGABA changes from -76.5 mV preFC to -46.1 mV postFC). Interestingly (see Discussion), the slope of the regression line for the observations obtained subsequent to FC is significantly larger (p<.003) than the slope of the best fitting line for the data obtained prior to FC.
Long-term enhancement of excitatory neurotransmission between small-diameter nociceptive afferents and the DHs neurons that receive their synaptic terminals (LTP) is regarded as the primary neural mechanism that underlies central sensitization and the associated abnormalities of pain perception, including hyperalgesia, allodynia, and persistent pain [3, 29]. Given the use dependent nature of LTP [24, 25], it is understandable that most studies have employed high-frequency afferent conditioning stimulation to induce it. Nevertheless, the demonstration  that LTP can be induced by spinal application of agents that evoke spike activity in DHs neurons makes it clear that presynaptic activity is not essential for LTP induction at the initial stage of the CNS pathways that process information about the status of peripheral nociceptors. Instead, the observations obtained in the present study make it likely  that LTP can result from the extrasynaptic spread of neuroactive compounds synthesized and released by DHs neurons or glia.
Although astrocytes normally function as "active partners" in CNS neurotransmission , a variety of evidence indicates that under a variety of pathological conditions (peripheral inflammation, injury, infection) their contributions can become maladaptive. The finding that nerve injury is accompanied by trans-synaptic reduction in the neuronal expression of the potassium-chloride exporter KCC2 - an alteration that disrupts lamina I neuron anion homeostasis - is notable in this regard because it raises the possibility that abnormal dorsal horn glianeuron interactions can contribute to hyperalgesia / persistent pain . Coull et al. (2003) demonstrated that DHs lamina I neuron expression of KCC2 is decreased after nerve injury - an outcome that results in the intracellular accumulation of Clin lamina I neurons which, in turn, shifts the Cl- equilibrium potential in a depolarizing direction and, in this way, converts the postsynaptic action of GABA from hyperpolarizing (inhibitory) to depolarizing (excitatory). As a result, after nerve injury DHs neurons exhibit abnormal excitability including a novel responsivity to peripheral stimuli that evoke activity in Aβ afferents [13, 14, 31]. Although comparable studies of DHs neurons remain to be carried out, Fiumelli et al. (2005) have demonstrated that repetitive postsynaptic spiking of hippocampal neurons leads (within minutes) to a Ca2+-dependent downregulation of KCC2 expression, a depolarizing shift of EGABA and, as a consequence, the effect of GABA converts from inhibition to excitation .
Although significant gaps (such as the effect of spike activity on KCC2) remain to be addressed, the findings obtained in the present study significantly extend current views of the mechanisms by which DHs excitatory neurotransmission undergoes modification. First, they demonstrate that the elevation of local [K+]o that occurs in the DHs subsequent to inhibition of astrocyte energy metabolism by FC is accompanied by a depolarizing shift in the EGABA of DHs neurons (Fig. 5B) - a shift which converts the effect of the transmembrane ionic currents associated with GABAA receptor activation convert from hyperpolarizing (inhibitory) to depolarizing (excitatory). Second, the observations obtained in the present study reveal that exposure to FC is accompanied by a significant increase in the slope of the relationship between membrane potential and the transmembrane current evoked by GABA (Fig. 5B) - an outcome indicating that DHs neurons exhibit a larger GABA-mediated membrane conductance to Cl- after exposure to FC (compare current traces obtained before and after FC; Fig. 5B).
The prominent suppression of the OISDR that reliably follows exposure to FC (Fig. 1) is fully consistent with the proposal that astrocytes are the major source of the tissue transmittance increase that underlies the intrinsic optical signal (OISDR) evoked in the DHs by dorsal root stimulation (reviewed in [4, 33, 34]). Such an effect of FC, taken together with the report  that in normal subjects [K+]o is maintained at lower values in the superficial dorsal horn (laminae I-II) than in the deeper laminae (laminae III-V), raises the functionally intriguing possibility that the capacity of astocytes to remove excess extracellular K+ (as well as glutamate and other neuroactive substances) is substantially greater in the DHs than in the deeper layers of the horn.
Although additional evidence will be required to confirm its validity, the view which guides our ongoing investigation of DHs glial-neural interactions is that astrocytes are part of a DHs control mechanism which, in normal subjects, permits relatively rapid and effective adjustment (accomplished via stimulus-directed astroctye-neuron interactions) of DHs neuron excitability/responsivity at this initial stage of CNS nociceptive information processing. The contributions to both normal and abnormal sensory function of such a control mechanism are regarded as potentially significant. More specifically, although the normally highly efficient astrocyte regulation of [K+]o in the DHs ensures the maintenance of a relatively low (presumably desirable) level of excitability at this initial level of the CNS "pain" projection path, the slow (relative to stimulus-driven neuronal activity) temporal characteristics of DHs astrocyte-mediated uptake and release of K+ and other neuroactive substances may contribute importantly to experience-driven and functionally-adaptive modulation of DHs neuron responsivity (e.g., these properties of DHs astrocytes may enable astrocytes to modify the "wind-up" behavior of DHs neurons). Finally, a vigorous episode of nociceptor afferent input (e.g., that evoked by intracutaneous injection of algesic chemical; ) is viewed to make this control system maladaptive by interfering with the ability of DHs astrocytes to regulate extracellular K+ and glutamate which, as demonstrated by the experimental findings described in this paper, triggers a long-term enhancement of DHs excitatory neurotransmission (LTP) which in a conscious subject, would be accompanied by perceptual abnormalities such as hyperalgesia, allodynia, and persistent pain.
Multiple animal behavioral studies have used FC to assess the role of glia in the hyperalgesia that occurs following manipulations such as intracutaneous injection of algesic chemical (e.g, formalin - ; zymosan - ), tetanic electrical stimulation of a peripheral nerve at C-fibre strength [37, 38], or perisciatic application of agents that evoke immune activation . Such studies have consistently reported that direct application of FC to the spinal cord (e.g., at 10 min - 1 hr before performance of the manipulation used to elicit hyperalgesia) at a dose that inhibits glial energy metabolism via the TCA cycle prevents or greatly attenuates the hyperalgesia - and this effect of FC has uniformly interpreted to indicate that the FC inhibition of glial metabolism prevents the glial release of a variety of factors critical for the excessive activation of dorsal horn neurons that underlies hyperalgesia.
This interpretation appears directly at odds with this study's finding that transient exposure to FC induces a long-term enhancement of excitatory neurotransmission in the superficial spinal cord dorsal horn. Consideration of our observations in the context of those reported in a recent study of hippocampal neuron-astrocyte metabolic interaction , however, suggests an alternative and, at least to date, not previously considered explanation for the finding that spinal pre-administration of FC prevents/attenuates the hyperalgesia induced by intracutaneous injection of algesic chemical, peri-sciatic immune activation, or tetanic stimulation of C-fibre afferents. That is, Bacci et al. (2002) found that exposure to FC not only inhibits astrocyte energy production via the TCA cycle, but also leads FC-intoxicated astrocytes to use glutamine as an alternative energy source, enabling astrocyte ATP levels to be maintained near-to-normal for hours (for concise review of effects of FC on astrocyte energy metabolism see ). As a result, as astrocyte release of glutamine declines in the presence of FC, primary afferent nerve terminals become deprived of the glutamine required to maintain the efficacy of excitatory glutaminergic neurotransmission between nociceptive afferents and DHs neurons, dorsal horn nociceptive information processing fails, and pain perception diminishes. Accordingly, although behavioral studies have shown that prior FC application to the spinal cord prevents/attenuates hyperalgesia, this effect of FC may not be attributable (as has been widely assumed) to astrocyte release of neuroactive substances, but instead may be the result of a decreased availability of astrocytic glutamine for conversion to glutamate in primary nociceptive afferent terminals (via the glutamine-glutamate cycle; [4, 40, 41]). The rapid onset of the DHs neuron LTP that occurs after washout of FC is regarded as consistent with this interpretation. In addition, our finding that LTPFC is reduced/eliminated by lowering K+ in the solution perfusing the slice (Fig. 2) is viewed to indicate that DHs [K+]o increases when astrocyte metabolism is inhibited, and contributes importantly (via direct actions on neurons and/or by enhancing NMDA receptor activity) to the long-term enhancement of DHs excitatory neurotransmission observed subsequent to exposure to FC.