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Exp Neurobiol 2020; 29(3): 207-218
Published online June 30, 2020
© The Korean Society for Brain and Neural Sciences
Woo Hyun Shin and Kwang Chul Chung*
Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea
Correspondence to: *To whom correspondence should be addressed.
TEL: 82-2-2123-2653, FAX: 82-2-312-5657
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The formation of Lewy bodies (LBs), intracellular filamentous inclusions, is one of the hallmarks of Parkinson's disease (PD). α-Synuclein is the main component of LBs and its abnormal accumulation contributes to the pathogenesis of PD. Direct phosphorylation of α-synuclein at multiple Ser/Tyr residues is known to induce its aggregation, consequently promoting LB formation. Death-associated protein kinase 1 (DAPK1), originally identified as a positive mediator of γ-interferon-induced programmed cell death, possesses tumor-suppressive activity and mediates a wide range of cellular processes, including apoptosis and autophagy. Accumulating evidence suggests that DAPK1 is also associated with neuronal cell death and neurodegeneration. For example, DAPK1 phosphorylates tau and amyloid precursor protein, and induces tau aggregation and amyloid β production, respectively, in Alzheimer’s disease. DAPK1 is also accumulated to a larger extent in a mouse model of PD, causing synucleinopathy and dopaminergic neuron degeneration. In this study, we attempted to determine whether DAPK1 phosphorylates α-synuclein and affects cell viability in human dopaminergic neuroblastoma SH-SY5Y cells. We demonstrated that DAPK1 directly phosphorylates α-synuclein at Ser129, and induces the formation of insoluble α-synuclein aggregates. We also showed that DAPK1 enhances rotenone-induced aggregation of α-synuclein, potentiating neuronal cell death. Taken together, these findings suggest that DAPK1 acts as a novel regulator of toxic α-synuclein aggregation, possibly affecting and playing a role in the development of PD.
Keywords: Death-associated protein kinase 1, α-Synuclein, Parkinson’s disease, Phosphorylation, Protein aggregation
Parkinson’s disease (PD) is one of the most common late-onset neurodegenerative diseases (NDDs), characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta . The formation of filamentous inclusion bodies called Lewy bodies (LBs) is the neuropathological feature of PD. The main component of these LBs is the protein α-synuclein. Under normal conditions, α-synuclein is present in soluble form. However, in deadly pathological conditions, α-synuclein forms insoluble aggregates [2,-4]. Moreover, α-synuclein undergoes a variety of post-translational modifications, including phosphorylation [5, 6], nitrosylation , ubiquitination [8, 9], and sumoylation , which impact α-synuclein oligomer formation and aggregation in different ways. In particular, hyperphosphorylation of α-synuclein promotes the formation of LBs . In healthy brains, only small fraction of α-synuclein is phosphorylated at S129 site (lower than 4%), but its dramatic accumulation (approximately 90%) has been observed in the brains of PD patients  or animal models of PD [13, 14].
Death-associated protein kinase 1 (DAPK1), a Ser/Thr kinase, acts as a positive regulator of apoptosis in response to various toxic stimuli, such as Fas, γ-interferon, and TNF-α [15,-17]. Overexpression of DAPK1 increases formation of autophagic vesicles during programmed cell death . Recent evidence suggests that DAPK1 regulates cell death in the brain, and that the alteration of kinase activity is closely associated with NDDs . For example, DAPK1 activation induces the phosphorylation of p53 at Ser23 , increasing apoptotic neuronal cell death [21, 22]. DAPK1 also regulates microtubule assembly, neuronal differentiation, and tau toxicity through the activation of microtubule-affinity regulating kinases 1 and 2 . DAPK1 protein levels were highly upregulated in the brain of Alzheimer’s disease (AD) patients . In addition, DAPK1 increased the phosphorylation of AD-related tau protein, consequently causing the accumulation of tau protein and toxicity . Furthermore, DAPK1 induces the phosphorylation of amyloid precursor protein (APP) at Thr668, and
In the present study, we examined the role of DAPK1 in α-synuclein phosphorylation, its aggregation, and cell viability in human dopaminergic neuronal cells. We found that DAPK1 directly binds to α-synuclein, phosphorylates α-synuclein at Ser129, and increases the formation of detergent-insoluble and toxic α-synuclein aggregates in SH-SY5Y cells. In addition, rotenone-induced aggregation of α-synuclein followed by neuronal death was considerably attenuated in
Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and Lipofectamine® LTX with PLUS reagent were purchased from Invitrogen (Carlsbad, CA, USA). Protein A-Sepharose beads were obtained from GE Healthcare Life Sciences (Piscataway, NJ, USA). Anti-c-Myc, anti-tubulin, anti-α-synuclein, and anti-GFP antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-FLAG and anti-DAPK1 antibodies were purchased from Sigma-Aldrich (St. Luis, MO, USA). Anti- phospho-α-synuclein (S129) antibody was purchased from Abcam (Cambridge, UK). Anti-phosphoserine antibody, peroxidase-conjugated goat anti-rabbit and anti-mouse secondary antibodies were purchased from Millipore (Billerica, MA, USA). Alexa Fluor® 488-conjugated anti-mouse and Alexa Fluor® 594-conjugated anti-rabbit secondary antibodies were purchased from Invitrogen. Enhanced chemiluminescence (ECL) reagent was purchased from Abclon (Seoul, Korea) and Advansta (Menlo Park, CA, USA). All other chemicals used in the study were analytical grade commercial products and purchased from Sigma-Aldrich.
Mammalian expression vectors encoding FLAG-tagged human wild-type DAPK1 (
Mouse embryonic ﬁbroblasts (MEFs) derived from
All mice were handled in accordance with the guideline for animal care and use of Yonsei University. Cerebral cortices were removed from gestational day 14.5 mouse embryos (Orient BIO, Seongnam-si, Gyeonggi-do, Republic of Korea) and mechanically dissociated. Briefly, dissociated cortical cells were plated at a density of 5×106 cells per well of six-well plates that were precoated with 100 µg/ml poly-D-lysine and 1 µg/ml laminin (Invitrogen). Cultures were incubated at 37°C in Minimum Essential Medium (MEM, Fisher Scientific, Waltham, MA, USA) supplemented with 0.6% glucose, 1 mM sodium pyruvate, 2 mM L-glutamine, penicillin-streptomycin, and 10% FBS in the atmosphere of 95% air and 5% CO2. After 24 h, culture medium was changed to Neurobasal medium (Invitrogen) supplemented with 2% B-27 and 0.5 mM L-glutamine. At 4 DIV, cultures were treated with the indicated drugs that were dissolved in the same medium.
Cell lysates containing ~500 µg protein were incubated overnight at 4°C with 0.5 µg of the appropriate antibodies. Samples were incubated with Protein A-Sepharose beads for 2 h at 4°C with rotation. The beads were then pelleted and washed five times with lysis buffer. The immunocomplexes were boiled in SDS-PAGE sample buffer, resolved by SDS-PAGE, and transferred to nitrocellulose membranes. Membranes were blocked in Tris-buffered saline with Tween 20 (TBST) containing 50 mM Tris (pH 7.4), 150 mM NaCl, 0.1% Tween 20, and 5% nonfat dry milk for 1 h at room temperature, and then incubated with the primary antibodies overnight at 4°C in 3% nonfat dry milk in TBST. The membranes were washed with TBST, incubated for 1 h with horseradish peroxidase (HRP)-coupled secondary IgG, washed again with TBST, and visualized using ECL reagents (Abclon, Seoul, Korea). Grayscale images of immunoblotting data were quantified by MultiGauge V. 3.1 program (Fujifilm Life Science, Tokyo, Japan).
Cells were seeded onto Poly-L-lysine coated glass cover slips. After DNA transfection for 24 h, the cells were washed twice with PBS (pH 7.4) and immediately fixed in 3.7% formaldehyde for 10 min at room temperature. After fixation, the cells were permeabilized with 0.2% Triton X-100 for 10 min and blocked with 1% bovine serum albumin in PBS for 1 h at room temperature. The cells were then immunostained with the appropriate primary antibody, and Alexa FluorTM 488-conjugated anti-mouse and Alexa FluorTM 594-conjugated anti-rabbit secondary antibodies were used to detect the primary antibodies. Images were captured using an LSM 700 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany), and data were processed using a Zeiss LSM Image Browser (Carl Zeiss). To quantitate the colocalization of DAPK1 and α-synuclein, Pearson’s correlation coefficients were calculated using Image J software (National Institutes of Health, USA).
Proximity ligation assay (PLA) was performed with Duolink PLA assay kit (Sigma-Aldrich), following to the manufacturer’s instruction.
SH-SY5Y cells were transfected for 24 h with plasmids encoding FLAG-tagged DAPK1-WT or its kinase-defective mutant (DAPK1-K42A). Cells were lysed in 1% NP-40 lysis buffer and cell lysates were immunoprecipitated overnight at 4°C with anti-FLAG antibody. The immunocomplexes were incubated with 30 µl of a 1:1 protein A-Sepharose bead suspension for 2 h at 4°C with gentle rotation. The beads were centrifuged and washed twice with lysis buffer and twice with kinase reaction buffer (40 mM Tris, pH 7.5, 20 mM MgCl2, and 50 µM DTT). The samples were mixed with 2 µg of bacterial recombinant GST-fused α-synuclein and kinase reaction buffer containing 10 µM ATP. The
The plasmids encoding GST-tagged α-synuclein were expressed in
Cells were washed twice with ice-cold PBS and lysed in lysis buffer containing 10 mM Tris (pH 7.4), 1% Triton X-100, 150 mM NaCl, 10% glycerol, 20 mM N-ethylmaleimide, and protease inhibitor cocktail. Cell lysates were centrifuged at 15,000 g for 20 min at 4°C. The supernatant was retained as the detergent-soluble fraction, and the pellet was washed twice with lysis buffer. The pellet was dissolved in lysis buffer supplemented with 4% SDS and boiled for 30 min (referred to as detergent-insoluble fraction). Each sample was resolved by SDS-PAGE and the protein bands were visualized by immunoblot analysis.
Cell extracts containing approximately 500 µg protein were applied onto cellulose acetate membranes with 0.2 mm-pores that had been prewashed with PBS containing 0.1% SDS. The membranes were then washed three times with PBS and stained with anti-Myc antibody. Sample staining was visualized by immunoblot analysis.
Native gel electrophoresis was performed with lysates containing ~20 µg protein, using the Native-PAGETM Bis-Tris 4%~16% precast minigel system (Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s instructions.
After DNA or siRNA transfection for 24 h, cells were left untreated or treated with rotenone for additional 24 h. The medium was removed and a 1:10 dilution of CCK-8 solution (Cell Counting Kit-8; Dojindo Laboratories, Kumamoto, Japan) in complete medium was added to the cells. Plates were incubated for 30 min at 37°C, and the absorbance at 450 nm was measured using a microplate reader.
All statistical analyses were performed using an unpaired Student’s t-test and the SPSS statistical analysis software (version 23.0) (IBM, Armonk, NY, USA). All values are expressed as the mean±standard error of the mean (SEM).
Based on the recent finding that DAPK1 acts as a novel regulator of synucleinopathy and dopaminergic neuron degeneration in a mouse model of PD , in the present study we examined whether DAPK1 mediates α-synuclein phosphorylation and aggregation in a dopaminergic neuronal cell line. We firstly examined whether there is a biochemical and functional interaction between DAPK1 and α-synuclein. To determine if DAPK1 directly binds to α-synuclein in neuronal cells, SH-SY5Y cells were transfected with plasmid encoding Myc-tagged α-synuclein or FLAG-tagged DAPK1 alone or together, and cell lysates were co-immunoprecipitated with anti-FLAG antibody. The immunoblot analysis of the samples with anti-FLAG antibodies revealed that ectopically expressed DAPK1 binds to α-synuclein (Fig. 1A). The biochemical interaction between endogenous DAPK1 and endogenous α-synuclein was also confirmed in SH-SY5Y cells (Fig. 1B). Moreover, interaction between endogenous DAPK1 and endogenous α-synuclein was verified in mouse primary cortical neurons (Fig. 1C). Furthermore, immunocytochemical analysis of SH-SY5Y cells revealed that overexpressed FLAG-DAPK1 and GFP-α-synuclein are colocalized, primarily in the cytosolic area, with a Pearson’s correlation coefficient of 0.58 (Fig. 1D). The colocalization of endogenous DAPK1 and α-synuclein was further confirmed with immunocytochemical analyses (Fig. 1E) and proximity ligation assay (Fig. 1F).
Because the phosphorylation of α-synuclein, especially at Ser129, is important for aggregate formation , we next examined whether DAPK1 directly phosphorylates α-synuclein on Ser129 in SH-SY5Y cells. Overexpression of DAPK1 caused a significant increase in the level of S129-phosphorylated α-synuclein. However, this effect was not seen with the DAPK1-K42A mutant (Fig. 2A), indicating that DAPK1 increases the phosphorylation of α-synuclein at Ser129 in a kinase-dependent manner.
Previous research indicates that rotenone treatment of SH-SY5Y cells induces phosphorylation of α-synuclein on Ser129, enhancing aggregation of α-synuclein . Based on this finding, we further examined whether the phosphorylation state of α-synuclein induced by rotenone is differentially affected in
To demonstrate that DAPK1 directly phosphorylates α-synuclein at Ser129, an
Taken together, these results indicated that DAPK1 directly phosphorylates α-synuclein at residue Ser129.
Because the phosphorylation of α-synuclein at S129 is known to stimulate the formation of its insoluble aggregate [3, 11], we next determined whether DAPK1 affects α-synuclein aggregation in SH-SY5Y cells. Firstly, filter trap assay revealed that the amount of α-synuclein aggregate was markedly increased by exogenous DAPK1 in a dose-dependent manner (Fig. 3A). However, overexpression of the DAPK1-K42A mutant had no effect on α-synuclein aggregation (Fig. 3A). Moreover, the α-synuclein-S129A mutant did not form notable aggregates, compared with wild-type α-synuclein (Fig. 3B). These results are consistent with the previous finding . Furthermore, aggregation of the α-synuclein-S129A mutant was unaffected by DAPK1-WT (Fig. 3B). Lastly, immunocytochemical analysis of SH-SY5Y cells revealed that DAPK1 overexpression increases the formation of intracellular α-synuclein inclusions (Fig. 3C), whereas DAPK1-K42A caused no measurable change in formation of inclusions (Fig. 3C). Especially, the immunocytochemical analysis of the samples with phospho-α-synuclein antibody (S129) revealed that DAPK1 overexpression increases the formation of pS129-α-synuclein-containing inclusions (Fig. 3D).
These results suggest that DAPK1 acts as positive regulator of α-synuclein aggregation.
Next, we determined whether DAPK1 acts as a novel regulator of rotenone-induced synucleinopathy in neuronal SH-SY5Y cells. Because rotenone treatment induces toxic aggregation of α-synuclein , we firstly checked whether DAPK1 affects the formation of high molecular weight α-synuclein aggregate in response to rotenone. SH-SY5Y cells were either mock-transfected or transfected with plasmid encoding FLAG-tagged DAPK1. After treatment with rotenone, cell lysates were sequentially extracted with lysis buffer containing 1% Triton-X100 (detergent-soluble fraction) and 4% SDS (detergent-insoluble fraction). Immunoblot of cell lysates with anti-α-synuclein antibody demonstrated that DAPK1 increases α-synuclein aggregation in the detergent-insoluble fraction (Fig. 4A). Then, we performed Native-PAGE to determine if DAPK1 affects the formation of rotenone-induced soluble oligomers of α-synuclein as well as aggregates. Whereas rotenone treatment induced formation of α-synuclein oligomer in the control
Finally, we examined whether DAPK1-mediated α-synuclein aggregation changes relative cell viability upon rotenone treatment in SH-SY5Y cells. CCK assay of the samples revealed that overexpression of DAPK1 alone does not significantly affect cell death (Fig. 4E). In addition, rotenone treatment causes toxic synucleinopathy in SH-SY5Y cells, increasing apoptotic cell death by more than 50% (Fig. 4E). Additionally, DAPK1 overexpression plus rotenone treatment triggered a synergistic increase in cell death in SH-SY5Y cells. However, this effect was not seen with the DAPK1-K42A mutant or with siRNA-mediated knockdown of α-synuclein (Fig. 4E).
These results suggest that DAPK1 acts as a novel regulator of rotenone-induced cell death in SH-SY5Y cells, potentiating cell death by enhancing toxic
DAPK1 is a mediator of pro-apoptotic pathways and is involved in various cell death processes induced by internal and external apoptotic stimuli [15, 17]. It regulates both caspase-dependent and autophagic, but caspase-independent, cell death signaling . Additionally, DAPK1 suppresses tumor growth and metastasis by promoting autophagy and apoptosis . In numerous cell culture models, DAPK1 has also been implicated in pathways leading to apoptosis and in some cases may function prior to the commitment steps of the signal transduction mechanism [22,28,-30]. Thus, some of the apoptotic properties of DAPK1 observed in cultured cells are likely responsible for certain aspects of human diseases. The present findings also verified the previous report that DAPK1 signaling induces synucleinopathy and dopaminergic neuron degeneration in a mouse model of PD .
α-Synuclein might be a target for a variety of post-translational modifications, including phosphorylation [5, 6], nitrosylation , ubiquitination [8, 9], and sumoylation , which may affect the biochemical properties and function of α-synuclein, such as the formation of its toxic aggregates. The most widely studied reaction is the phosphorylation of α-synuclein that occurs at Ser129 . Within the α-synuclein protein there are four Ser residues that are potential phosphorylation sites, of which Ser129 is the most important and influential for LB formation and PD pathogenesis . Phosphorylation of α-synuclein at Ser129 is mediated and regulated by various kinases. For example, casein kinase and polo-like kinase are representative kinase that facilitate α-synuclein phosphorylation at Ser129 [32,-34]. In addition to these enzymes, the present study identified another kinase of α-synuclein, DAPK1, that mediates Ser129-phosphorylation. In addition to oligomerization and aggregation, phosphorylation of Ser129 alters the physiological functions of α-synuclein in mammalian cells. For example, the binding of α-synuclein to membranes was significantly weakened by Ser129-phosphorylation [35, 36]. The subcellular localization of α-synuclein was also changed by phosphorylation, resulting in the increased presence of α-synuclein within the nuclear side . Interestingly, ~90% of α-synuclein found in LBs is phosphorylated , and phosphorylation of α-synuclein at Ser129 is associated with neurodegeneration in the rat PD model [39, 40]. Our data further support the hypothesis that DAPK1 plays an important role in α-synuclein-mediated neurodegeneration, and possibly in the pathogenesis of PD.
DAPK1 is widely known as a tumor suppressor that modulates cell survival and death in a variety of cell systems. Moreover, accumulating evidence has shown that apoptotic neuronal death is regulated by DAPK1, and defective DAPK1 function is associated with NDDs [19, 21, 22, 30]. For example, DAPK1 plays a role in brain stroke by interacting with NMDA receptors . In addition, DAPK1 activity negatively regulates spatial memory in mice . In AD, DAPK1 increases the phosphorylation of AD-related tau, thereby increasing the accumulation of tau inclusions and toxicity . DAPK1 also induces the phosphorylation of APP at Thr668, whereas
The formation of insoluble aggregates and LBs is a good indicator of the neuronal toxicity of α-synuclein. As hyperphosphorylated α-synuclein accumulated excessively and makes up the majority of α-synuclein in LBs , the increase in α-synuclein phosphorylation by DAPK1 would be expected to augment the formation of α-synuclein aggregates. This hypothesis was confirmed by the filter trap and native PAGE analyses, showing that DAPK1 increases the amount of α-synuclein aggregates in SH-SY5Y cells through α-synuclein phosphorylation at Ser129. Moreover, DAPK1-mediated phosphorylation was shown to be essential to the formation of intracellular α-synuclein inclusions. Lastly, we demonstrated that the production of insoluble α-synuclein aggregates by DAPK1 exacerbates apoptotic SH-SY5Y cell death under conditions of rotenone treatment.
In conclusion, the present study revealed that DAPK1 plays an important role in stimulating toxic α-synuclein aggregation and neuronal cell death.
The authors thank T.H. Lee for providing plasmids,