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Original Article

Exp Neurobiol 2011; 20(2): 110-115

Published online June 30, 2011

© The Korean Society for Brain and Neural Sciences

Simple and Novel Three Dimensional Neuronal Cell Culture Using a Micro Mesh Scaffold

Sang Jin Yoo1, Jongmin Kim2, Chang-Soo Lee2 and Yoonkey Nam1*

1Department of Bio and Brain Engineering, KAIST, Daejeon 305-701, 2Department of Chemical Engineering, Chungnam National University, Daejeon 305-764, Korea

Correspondence to: *To whom correspondence should be addressed.
TEL: 82-42-350-4362, FAX: 82-42-350-4310


Conventional method of cell culture studies has been performed on two-dimensional substrates. Recently, three-dimensional (3D) cell culture platforms have been a subject of interest as cells in 3D has significant differences in cell differentiation and behavior. Here we report a novel approach of 3D cell culture using a nylon micro mesh (NMM) as a cell culture scaffold. NMM is commonly used in cell culture laboratory, which eliminates the requirement of special technicality for biological laboratories. Furthermore, it is made of a micro-meter thick nylon fibers, which was adequate to engineer in cellular scales. We demonstrate the feasibility of the NMM as a 3D scaffold using E18 rat hippocampal neurons. NMM could be coated with cell adhesive coatings (polylysine or polyelectrolyte) and neurons showed good viability. Cells were also encapsulated in an agarose hydrogel and cultured in 3D using NMM. In addition, the 3D pattern of NMM could be used as a guidance cue for neurite outgrowth. The flexible and elastic properties of NMMs made it easier to handle the scaffold and also readily applicable for large-scale tissue engineering applications.

Keywords: 3D culture, scaffold, micro mesh, neuronal culture, 3D cell patterning