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Exp Neurobiol 2011; 20(4): 169-175
Published online December 30, 2011
https://doi.org/10.5607/en.2011.20.4.169
© The Korean Society for Brain and Neural Sciences
Eun Hee Ahn1#, Dae Won Kim1#, Min Jea Shin1, Soon Won Kwon1, Young Nam Kim1, Duk-Soo Kim2, Soon Sung Lim3, Joon Kim4, Jinseu Park1, Won Sik Eum1, Hyun Sook Hwang1* and Soo Young Choi1*
1Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, 2Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 330-090, 3Department of Food Science and Nutrition & RIC Center, Hallym University, Chunchon 200-702, 4School of Life Science and Biotechnology, Korea University, Seoul 136-701, Korea
Correspondence to: #These authors equally contributed to this work.
*To whom correspondence should be addressed.
TEL: 82-33-248-2112, FAX: 82-33-248-3201
e-mail: Soo Young Choi sychoi@hallym.ac.kr and
Hyun Sook Hwang wazzup@hallym.ac.kr
Chlorogenic acid (CGA) possesses various biological activities such as anti-oxidant, anti-inflammatory, and anti-diabetic activities. In the present study, we examined the effect of CGA on the transduction efficiency of PEP-1-ribosomal protein S3 (PEP-1-rpS3) into cells and brain tissues, and its neuroprotective potential against ischemia/reperfusion. We found that, in the presence of CGA, the transduction efficiency of PEP-1-rpS3 into astrocytes and the CA1 region of the hippocampus was enhanced, compared to its transduction in the absence of CGA. Also, cell viability data demonstrated that the sample treated with CGA + PEP-1-rpS3 exhibited improved cell viability against hydrogen peroxide (H2O2)-induced toxicity more significantly than the sample treated with PEP-1-rpS3 alone. Also, in a gerbil ischemia model, data demonstrated that following the ischemic insult, the group treated with PEP-1-rpS3 + CGA showed markedly enhanced protection of neuron cells in CA1 region of hippocampus, compared to those treated with CGA or PEP-1-rpS3 alone. Taken together, these results suggest that CGA may improve the transduction efficiency of protein transduction domain (PTD) fusion proteins into target cells or tissues, thereby enhancing their therapeutic potential against various diseases.
Keywords: chlorogenic acid, PEP-1-ribosomal protein S3, protein transduction, ischemic insult