Articles

  • the Korean Society for Brain and Neural Sciences

Article

Review Article

Exp Neurobiol 2012; 21(3): 94-100

Published online September 30, 2012

https://doi.org/10.5607/en.2012.21.3.94

© The Korean Society for Brain and Neural Sciences

Medaka Fish Parkinson's Disease Model

Hideaki Matsui1*, Roberto Gavinio2 and Ryosuke Takahashi2*

1Department of Cell Physiology, Zoological Institute, Technical University Brauschweig, Braunschweig 38106, Germany, 2Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan

Correspondence to: *To whom correspondence should be addressed.
Hideaki Matsui
TEL: 49-531-391-3235, FAX: 49-531-391-3222
e-mail: hide0729@kuhp.kyoto-u.ac.jp
Ryosuke Takahashi
TEL: 81-75-751-3770, FAX: 81-75-761-9780
e-mail: ryosuket@kuhp.kyoto-u.ac.jp

Abstract

The teleost fish has been widely used in creating neurodegenerative models. Here we describe the teleost medaka fish Parkinson's disease (PD) models we developed using toxin treatment and genetic engineering. 1-Methyl-4-phenyl-1,2,3,4-tetrahydropyridine (MPTP), 6-hydroxydopamine (6-OHDA), proteasome inhibitors, lysosome inhibitors and tunicamycin treatment in our model fish replicated some salient features of PD: selective dopamine cell loss and reduced spontaneous movement with the last three toxins producing inclusion bodies ubiquitously in the brain. Despite the ubiquitous distribution of the inclusion bodies, the middle diencephalic dopaminergic neurons were particularly vulnerable to these toxins, supporting the idea that this dopamine cluster is similar to the human substantia nigra. PTEN-induced putative kinase 1 (PINK1) homozygous mutants also showed reduced spontaneous swimming movements. These data indicate that medaka fish can serve as a new model animal of PD. In this review we summarize our previous data and discuss future prospects.

Keywords: Parkinson's disease, medaka fish, dopaminergic neurons, model animal