Exp Neurobiol 2015; 24(1): 55-70
Published online March 31, 2015
© The Korean Society for Brain and Neural Sciences
Hyo Eun Moon1,2,3#, Kyunghee Byun4,5#, Hyung Woo Park1,2,3, Jin Hyun Kim6, Jin Hur7, Joong Shin Park8, Jong Kwan Jun8, Hyo-Soo Kim7, Seung Leal Paek1,2,3,9, In Keyoung Kim1, Jae Ha Hwang1, Jin Wook Kim1, Dong Gyu Kim1, Young Chul Sung10, Gou-Young Koh11, Chang W Song12, Bonghee Lee4,5* and Sun Ha Paek1,2,3*
1Department of Neurosurgery, 2Cancer Research Institute, 3Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-744, 4Center for Regenerative Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, 5Department of Anatomy and Cell Biology, Gachon University Medical School, Incheon 406-840, 6Clinical Research Institute, Gyeongsang National University Hospital, Jinju 660-702, 7Innovative Research Institute for Cell Therapy (IRICT), Seoul National University Hospital, 8Department of Obstetrics & Gynecology, Seoul National University Hospital, Seoul 110-744, Korea, 9Department of Neurosurgery, Mayo Clinic, USA, 10Division of Molecular and Life Science, Integrative Bioscience & Biotechnology, Pohang University of Science and Technology, Pohang 790-784, 11Laboratory for Vascular Biology and Stem Cell, Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-338, Korea, 12Department of Therapeutic Radiology-Radiation Oncology, University of Minnesota Medical School, Minneapolis, USA
Correspondence to: *To whom correspondence should be addressed.
Sun Ha Paek, TEL: 82-2-2072-3993, FAX: 82-2-744-8459, e-mail: firstname.lastname@example.org
Bonghee Lee, TEL: 82-32-899-6582, FAX: 82-32-899-6519, e-mail: email@example.com
#These authors equally contributed to this work.
Successful recovery from brain ischemia is limited due to poor vascularization surrounding the ischemic zone. Cell therapy with strong angiogenic factors could be an effective strategy to rescue the ischemic brain. We investigated whether cartilage oligomeric matrix protein (COMP)-Ang1, a soluble, stable and potent Ang1 variant, enhances the angiogenesis of human cord blood derived endothelial progenitor cells (hCB-EPCs) for rescuing brain from ischemic injury. COMP-Ang1 markedly improved the tube formation of capillaries by EPCs and incorporation of EPCs into tube formation with human umbilical vein endothelial cells (HUVECs) upon incubation on matrigel
Keywords: COMP-Ang1, angiogenesis, ischemia, Tie2-FAK-AKT pathway