Original Article

Exp Neurobiol 2017; 26(2): 82-89

Published online April 30, 2017

© The Korean Society for Brain and Neural Sciences

Immunohistochemical Localization of Translationally Controlled Tumor Protein in Axon Terminals of Mouse Hippocampal Neurons

Seong-Yeon Bae1†, Vadim Sheverdin1†, Jeehye Maeng1, In Kyoon Lyoo2, Pyung-Lim Han3* and Kyunglim Lee1*

1Graduate School of Pharmaceutical Sciences, College of Pharmacy, 2Ewha Brain Institute, Department of Brain and Cognitive Sciences, College of Pharmacy, Graduate School of Pharmaceutical Sciences, 3Department of Brain and Cognitive Sciences, Brain Disease Research Institute, and Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Korea

Correspondence to: *To whom correspondence should be addressed.
Kyunglim Lee, TEL: 82-2-3277-3024, FAX: 82-2-3277-2851
Pyung-Lim Han, TEL: 82-2-3277-4130, FAX: 82-2-3277-3419
These authors contributed equally to this work.

Received: January 24, 2017; Revised: March 1, 2017; Accepted: March 1, 2017


Translationally controlled tumor protein (TCTP) is a cytosolic protein with microtubule stabilization and calcium-binding activities. TCTP is expressed in most organs including the nervous system. However, detailed distribution and functional significance of TCTP in the brain remain unexplored. In this study, we investigated the global and subcellular distributions of TCTP in the mouse brain. Immunohistochemical analyses with anti-TCTP revealed that TCTP was widely distributed in almost all regions of the brain including the cerebral cortex, thalamus, hypothalamus, hippocampus, and amygdala, wherein it was localized in axon tracts and axon terminals. In the hippocampus, TCTP was prominently localized to axon terminals of the perforant path in the dentate gyrus, the mossy fibers in the cornu ammonis (CA)3 region, and the Schaffer collaterals in the CA1 field, but not in cell bodies of granule cells and pyramidal neurons, and in their dendritic processes. Widespread distribution of TCTP in axon tracts and axon terminals throughout the brain suggests that TCTP is likely involved in neurotransmitter release and/or maintaining synaptic structures in the brain, and that it might have a role in maintaining synaptic functions and synaptic configurations important for normal cognitive, stress and emotional functions.

Keywords: Cognition, Immunohistochemistry, Mossy fiber, Mouse hippocampus, Translationally Controlled Tumor Protein (TCTP)