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Exp Neurobiol 2017; 26(4): 206-212

Published online August 31, 2017

© The Korean Society for Brain and Neural Sciences

Decreased Glial GABA and Tonic Inhibition in Cerebellum of Mouse Model for Attention-Deficit/ Hyperactivity Disorder (ADHD)

Yoo Sung Kim1†, Junsung Woo2,3†, C. Justin Lee2,3,4 and Bo-Eun Yoon1*

1Department of Molecular biology, Dankook University, Cheonan, Chungnam 31116, 2Center for Neuroscience and Functional Connectomics, Korea Institute of Science and Technology (KIST), Seoul 02792, 3Neuroscience Program, University of Science and Technology (UST), Daejeon 34113, 4KU-KIST Graduate School of Converging Sciences and Technologies, Korea University, Seoul 02841, Korea

Correspondence to: *To whom correspondence should be addressed.
TEL: 82-41-550-3694, FAX: 82-41-559-7941
These authors contributed equally

Received: July 24, 2017; Revised: August 23, 2017; Accepted: August 23, 2017


About 5~12% of school-aged children suffer from the Attention-Deficit/Hyperactivity Disorder (ADHD). However, the core mechanism of ADHD remains unclear. G protein-coupled receptor kinase-interacting protein-1 (GIT1) has recently been reported to be associated with ADHD in human and the genetic deletion of GIT1 result in ADHD-like behaviors in mice. Mice lacking GIT1 shows a shift in neuronal excitation/inhibition (E/I) balance. However, the pricise mechanism for E/I imbalance and the role of neuron-glia interaction in GIT1 knockout (KO) mice have not been studied. Especially, a possible contribution of glial GABA and tonic inhibition mediated by astrocytic GABA release in the mouse model for ADHD remains unexplored. Therefore, we investigated the changes in the amount of GABA and degree of tonic inhibition in GIT1 KO mice. We observed a decreased glial GABA intensity in GIT1 KO mice compared to wild type (WT) mice and an attenuation of tonic current from cerebellar granule cells in GIT1 KO mice. Our study identifies the previously unknown mechanism of reduced astrocytic GABA and tonic inhibition in GIT1 lacking mice as a potential cause of hyperactivity disorder.

Keywords: ADHD, GIT1, tonic inhibition, glia, astrocyte, GABA