Exp Neurobiol 2017; 26(6): 369-379
Published online December 31, 2017
© The Korean Society for Brain and Neural Sciences
Jin-Young Park1, Juli Choi1, Yunjin Lee1, Jung-Eun Lee1, Eun-Hwa Lee1, Hye-Jin Kwon1, Jinho Yang2, Bo-Ri Jeong2, Yoon-Keun Kim2 and Pyung-Lim Han1,3*
1Department of Brain and Cognitive Sciences, Ewha Womans University, Seoul 03760, 2MD Healthcare Inc., Seoul 03923, 3Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Korea
Correspondence to: *To whom correspondence should be addressed.
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Emerging evidence has suggested that the gut microbiota contribute to brain dysfunction, including pathological symptoms of Alzheimer disease (AD). Microbiota secrete membrane vesicles, also called extracellular vesicles (EVs), which contain bacterial genomic DNA fragments and other molecules and are distributed throughout the host body, including blood. In the present study, we investigated whether bacteria-derived EVs in blood are useful for metagenome analysis in an AD mouse model. Sequence readings of variable regions of 16S rRNA genes prepared from blood EVs in Tg-APP/PS1 mice allowed us to identify over 3,200 operational taxonomic units corresponding to gut microbiota reported in previous studies. Further analysis revealed a distinctive microbiota landscape in Tg-APP/PS1 mice, with a dramatic alteration in specific microbiota at all taxonomy levels examined. Specifically, at the phylum level, the occupancy of