• the Korean Society for Brain and Neural Sciences


Short Communication

Exp Neurobiol 2018; 27(3): 238-244

Published online June 30, 2018

© The Korean Society for Brain and Neural Sciences

Leucine-rich G Protein-coupled Receptor-5 Is Significantly Increased in the Aqueous Humor of Human Eye with Proliferative Diabetic Retinopathy

Eun Hee Hong1†, Mina Hwang2†, Yong Un Shin1, Hyun-Hee Park2, Seong-Ho Koh2* and Heeyoon Cho1*

1Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Korea, 2Department of Neurology, Hanyang University College of Medicine, Seoul 04763, Korea

Correspondence to: *To whom correspondence should be addressed.
Heeyoon Cho, TEL: 82-31-560-2350, FAX: 82-31-551-6267
Seong-Ho Koh, TEL: 82-31-560-2260, FAX: 82-31-560-2267
These authors contributed equally.

Received: April 13, 2018; Revised: June 8, 2018; Accepted: June 21, 2018

Leucine-rich G protein-coupled receptor-5 (LGR5) is known to be a stem cell marker in many organs. LGR5 may have important roles in proliferative diabetic retinopathy (PDR) because LGR5 potentiate the Wnt/β-catenin pathway, which plays crucial roles in pathologic neovascularization in the retina. The association between LGR5 and retinal pathologic neovascularization has not yet been reported. In the present study, LGR5 was compared in human aqueous humor (AH) between normal control and patients with PDR to confirm the relationship between LGR5 and PDR. AH was collected from 7 naïve PDR patients and 3 control subjects before intravitreal injection and cataract surgery, respectively. LGR5 and key members of Wnt/β-catenin were assessed by western blotting. In the present study, it was confirmed for the first time that LGR5 is detected in AH and it increases in PDR patients. Key members of Wnt/β-catenin pathway were also increased in AH of PDR patients compared to control. These findings might support the hypothesis that LGR5 has important roles in PDR especially considering the roles of the Wnt/β-catenin pathway, which is activated by LGR5, contributing to retinal pathologic neovascularization.

Graphical Abstract

Keywords: Leucine-rich G protein-coupled receptor-5, Aqueous humor, Pathologic neovascularization, Diabetic retinopathy, Wnt signaling pathway