Articles

Article

Original

Exp Neurobiol 2003; 12(1): 19-23

Published online July 1, 2003

© The Korean Society for Brain and Neural Sciences

Blockade of the Hyperpolarization-activated Inward Current by Ethanol in Cerebellar Purkinje Cells

Wha Sook Seo1,*, Sook-Young Lee2,Sang Yong Jung2 and Chang Kook Suh2

Departments of 1Nursing and 2Physiology & Biophysics,College of Medicine, Inha University, Incheon, South Korea

Correspondence to: *To whom correspondence should be addressed.
TEL: 82-32-860-8203, FAX: 82-32-874-5880
e-mail: wschang@inha.ac.kr

Abstract

This study was performed to examine the acute effect of ethanol (EtOH) on the hyperpolarization-activated inward current (Ih), one of the currents that is considered to control the excitability and firing patterns of Purkinje cells (PCs) using an intracellular recording method in slice preparation of rat cerebellum. The result showed that the amplitude of depolarizing sag and rebound depolarization, which have been known to be mediated by Ih, were reduced in 68.8% and 66.7% of PCs tested, respectively. In a voltage clamp study, a slowly activating inward relaxation was also decreased with EtOH application in 57.1% of PCs tested.

Keywords: Ethanol, hyperpolarization-activated inward current (Ih), cerebellar purkinje cells, rebound depolarization